Mouse anti-Human Thy1/CD90 Monoclonal Antibody | anti-THY1 antibody

Anti-Thy1/CD90 Antibody (PE), Mouse Monoclonal

Cat. Num. AAA8107766

• Gene Names: THY1; CD90
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: Thy1/CD90; Monoclonal Antibody; Anti-Thy1/CD90 Antibody (PE); Mouse Monoclonal; Thy1 Antibody (PE); Mouse MAb; Thy-1 cell surface antigen; Anti-CD90 Antibody; anti-THY1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 10
• Specificity: Human Thy1
• Purity/Purification: Protein A
• Form/Format: Liquid; Aqueous solution containing 0.5% BSA and 0.03%ProClin300
• Concentration: 5ul/Test, 0.1mg/ml (varies by lot)

• Applicable Applications for anti-THY1 antibody: Flow Cytometry (FC/FACS/FCM)
• Immunogen: Recombinant Human Thy1 Protein
• Conjugation: PE
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Thy1 (rh Thy1) and conjugated with PE under optimum conditions, the unreacted PE was removed.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze!

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Human THY-1(CD90) expression on HEL92.1.7 cells. Cells were stained with PE-conjugated anti-Human THY-1(CD90). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)

Related Product Information for anti-THY1 antibody

Thy-1 membrane glycoprotein, also known as Thy-1 antigen, CD90 and THY1, is a cell membrane protein which contains 1 Ig-like V-type (immunoglobulin-like) domain. It is a glycophosphatidylinositol-linked glycoprotein expressed on the surface of neurons, thymocytes, subsets of fibroblasts, endothelial cells, mesangial cells and some hematopoietic cells. It has been identified on a variety of stem cells and at varying levels in non-lymphoid tissues such as on fibroblasts, brain cells, and activated endothelial cells. Thy-1 is evolutionarily conserved, developmentally regulated, and often has dramatic effects on cell phenotype. Thy-1 is a 25-37 kDa glycosylphosphatidylinositol (GPI)-anchored protein involved in T cell activation, neurite outgrowth, apoptosis, tumor suppression, wound healing, and fibrosis. To mediate these diverse effects, Thy-1 participates in multiple signaling cascades. Thy-1 is an important regulator of cell-cell and cell-matrix interactions, with important roles in nerve regeneration, metastasis, inflammation, and fibrosis.

References

Rege TA, et al. (2006) Thy-1 as a regulator of cell-cell and cell-matrix interactions in axon regeneration, apoptosis, adhesion, migration, cancer, and fibrosis. FASEB J. 20(8): 1045-54.Fiegel HC, et al. (2008) Lack of Thy1 (CD90) expression in neuroblastomas is correlated with impaired survival. Pediatr Surg Int. 24(1): 101-5.Bradley JE, et al. (2009) Roles and regulation of Thy-1, a context-dependent modulator of cell phenotype. Biofactors. 35(3): 258-65.Kisselbach L, et al. (2009) CD90 Expression on human primary cells and elimination of contaminating fibroblasts from cell cultures. Cytotechnology. 59(1): 31-44.

NCBI and Uniprot Product Information

• NCBI GI #: 19923362
• NCBI GeneID: 7070
• NCBI Accession #: NP_006279.2
• NCBI GenBank Nucleotide #: NM_006288.3
• UniProt Accession #: P04216; Q16008; Q9NSP1
• Molecular Weight: 17,935 Da
• NCBI Official Full Name: thy-1 membrane glycoprotein preproprotein
• NCBI Official Synonym Full Names: Thy-1 cell surface antigen
• NCBI Official Symbol: THY1
• NCBI Official Synonym Symbols: CD90
• NCBI Protein Information: thy-1 membrane glycoprotein; CDw90; thy-1 antigen; Thy-1 T-cell antigen
• UniProt Protein Name: Thy-1 membrane glycoprotein
• Protein Family: Thy-1 membrane glycoprotein
• UniProt Gene Name: THY1
• UniProt Entry Name: THY1_HUMAN

NCBI Description

This gene encodes a cell surface glycoprotein and member of the immunoglobulin superfamily of proteins. The encoded protein is involved in cell adhesion and cell communication in numerous cell types, but particularly in cells of the immune and nervous systems. The encoded protein is widely used as a marker for hematopoietic stem cells. This gene may function as a tumor suppressor in nasopharyngeal carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

Uniprot Description

THY1: May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

Protein type: Motility/polarity/chemotaxis; Membrane protein, GPI anchor; Cell adhesion

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: anchored to external side of plasma membrane; focal adhesion; growth cone; endoplasmic reticulum; integral to plasma membrane; dendrite; apical plasma membrane; plasma membrane; cytosol; lipid raft; external side of plasma membrane

Molecular Function: integrin binding; protein binding; protein kinase binding

Biological Process: focal adhesion formation; cell-cell adhesion; retinal cone cell development; negative regulation of T cell receptor signaling pathway; negative regulation of protein kinase activity; negative regulation of axonogenesis; cytoskeleton organization and biogenesis; angiogenesis; positive regulation of T cell activation; positive regulation of release of sequestered calcium ion into cytosol; T cell receptor signaling pathway; negative regulation of cell migration; positive regulation of GTPase activity