Testing Data
CD152 protein
CD152(CTLA-4)-muIg-R-PE
Reactivity
Human, Primate, Mouse, Pig, Cow, Cat
Applications
Flow Cytometry, Functional Assay, ELISA
Synonyms
CD152; CD152(CTLA-4)-muIg-R-PE; Human CD152(CTLA-4)-muIg/R-PE Fusion Protein (also binds to mouse CD80/CD86); CD152 protein
Host
BHK cells
Reactivity
Human, Primate, Mouse, Pig, Cow, Cat
Form/Format
50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA.
Concentration
Volume: 200 ul (varies by lot)
Applicable Applications for CD152 protein
Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes
1:20 (or use 4 mul of concentrated stock per 5 x 105-cell test)
Molecular Structure
A soluble 110 kd dimeric fusion protein consisting of the extracellular (125aa) domain of human CD152 (CTLA-4) fused to murine IgG2a Fc
Transfectant Cell Line
BHK
Performance
Five x 105 cultured Raji human tumor cells were washed and incubated 45 minutes on ice with 80 mul of CD152-muIg/R-PE at a dilution factor of 1:20 (10 mug/ml). Cells were washed three times, fixed and analyzed using by FACS. The cells stained positive with a mean shift of 1.97 log10 fluorescent units when compared to a Mouse IgG2a/R-PE negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 mul of a mixture of 0.5 mg/ml each anti-CD80, and anti-CD86 antibodies.
Conjugation
PE
Production
Human CD152-muIg fusion protein from tissue culture supernatant of BHK transfectants was Protein A purified to >95% by SDS-PAGE (<1% bovine immunoglobulin), and conjugated to R-Phycoerythrin through a sulfo-ester linkage. Unconjugated fusion protein was removed using size exclusion chromatography. CD152-muIg/R-PE conjugate is at 0.2 mg/ml with an A565/A280 ratio of 3.97.
Buffer
50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative)
Preparation and Storage
Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Protect from light
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Testing Data
Testing Data
Related Product Information for CD152 protein
Information: Immune response mediated by T cells can be characterized to functionally proceed as follows: antigen recognition by the T cell receptor, activation through costimulation, effector activities to eliminate antigen and finally down regulation. Human CD152 is a cell surface glycoprotein expressed at low levels on activated T cells. CD152 is a high affinity receptor for the costimulatory molecules CD80 (B7-1) and CD86 (B7-2) and appears to function as a negative regulator of T cell activation. Therefore, CD152 may be an important player in down regulating T cell mediated immune responses. The CD152 Ig fusion protein has biological activity and binds with high affinity to human or mouse CD80 (B7-1) and CD86 (B7-2). CD152 Ig will block the binding of anti-CD80 (B7-1) and anti-CD86 (B7-2) monoclonal antibodies.
References
T. Lindsten, et al, (1993) J Immunol 151: 3489-3499. T.L. Walunas, et al, (1994) Immunity 1: 405-413. N.J. Karandikar, et al, (1996) J Exp Med 184: 783-788. A.H. Cross, et al, (1995) J Clin Invest 95: 2783-2789. P.A. Morton,
et al, (1996) J Immunol 156: 1047-1054. Martin K. Oaks and Karen M. Hallett, (2000) J Immunol 164: 5015-5018.
S.J. Fass, et al, (2000) J Immunol 164: 6340-6348.
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Product Notes
The CD152 (Catalog #AAA666880) is a Protein produced from BHK cells and is intended for research purposes only. The product is available for immediate purchase. The CD152(CTLA-4)-muIg-R-PE reacts with Human, Primate, Mouse, Pig, Cow, Cat and may cross-react with other species as described in the data sheet. AAA Biotech's CD152 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA). 1:20 (or use 4 mul of concentrated stock per 5 x 105-cell test). Researchers should empirically determine the suitability of the CD152 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD152, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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