Rabbit anti-Human, Mouse Beclin-1 Polyclonal Antibody | anti-BECN1 antibody

Beclin-1 Polyclonal Antibody

Cat. Num. AAA8527336

• Gene Names: BECN1; ATG6; VPS30; beclin1
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Affinity purification
• Synonyms: Beclin-1; Polyclonal Antibody; Beclin-1 Polyclonal Antibody; ATG6; VPS30; beclin1; anti-BECN1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 450

• Applicable Applications for anti-BECN1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: WB: 1:200-1:500; IHC: 1:50-1:100; IP: 1:20-1:50
• Immunogen: Recombinant protein of human Beclin-1
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of A431 cellline, using Beclin-1antibody.)

Related Product Information for anti-BECN1 antibody

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of proteins activated in response to nutrient deprivation and in neurodegenerative conditions (1). One of the proteins critical to this process is Beclin-1, the mammalian orthologue of the yeast autophagy protein Apg6/Vps30 (2). Beclin-1 can complement defects in yeast autophagy caused by loss of Apg6 and can also stimulate autophagy when overexpressed in mammalian cells (3). Mammalian Beclin-1 was originally isolated in a yeast two-hybrid screen for Bcl-2 interacting proteins and has been shown to interact with Bcl-2 and Bcl-xL but not with Bax or Bak (4). While Beclin-1 is generally ubiquitously expressed, it is monoallelically deleted in 40-75% of sporadic human breast and ovarian cancers (5). It is localized within cytoplasmic structures including the mitochondria, although overexpression of Beclin-1 reveals some nuclear staining and CRM1-dependent nuclear export (6). Beclin-1-/-mice die early in embryogenesis and Beclin-1-/+ mice have a high incidence of spontaneous tumors. Stem cells from the null mice demonstrate an altered autophagic response although responses to apoptosis appeared normal (7). Overexpression of Beclin-1 in virally infected neurons in vivo resulted in significant protection against Sindbis virus-induced disease and neuronal apoptosis (4).

References

1. Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21. 2. Kametaka, S. et al. (1998) J Biol Chem 273, 22284-91. 3. Liang, X.H. et al. (1999) Nature 402, 672-6. 4. Liang, X.H. et al. (1998) J Virol 72, 8586-96. 5. Aita, V.M. et al. (1999) Genomics 59, 59-65. 6. Liang, X.H. et al. (2001) Cancer Res 61, 3443-9. 7. Yue, Z. et al. (2003) Proc Natl Acad Sci U S A 100, 15077-82.

NCBI and Uniprot Product Information

• NCBI GI #: 929524267
• NCBI GeneID: 8678
• NCBI Accession #: NP_001300927.1
• NCBI GenBank Nucleotide #: NM_001313998.1
• UniProt Accession #: Q14457; O75595; Q9UNA8; B2R6N7
• Molecular Weight: 52kDa
• NCBI Official Full Name: beclin-1 isoform a
• NCBI Official Synonym Full Names: beclin 1
• NCBI Official Symbol: BECN1
• NCBI Official Synonym Symbols: ATG6; VPS30; beclin1
• NCBI Protein Information: beclin-1
• UniProt Protein Name: Beclin-1
• Protein Family: Beclin
• UniProt Gene Name: BECN1
• UniProt Synonym Gene Names: GT197

NCBI Description

This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

Plays a central role in autophagy (PubMed:23184933, PubMed:28445460). Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123, PubMed:20208530, PubMed:26783301). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521). Protects against infection by a neurovirulent strain of Sindbis virus (PubMed:9765397). May play a role in antiviral host defense.