CyclinE2 Recombinant Protein | CYCE2 recombinant protein

CyclinE2 Protein

Cat. Num. AAA515486

• Gene Names: CCNE2; CYCE2
• Applications: Western Blot
• Synonyms: CyclinE2; CyclinE2 Protein; CYCE2 recombinant protein

• Host: Sf9 insect cells using baculovirus
• Form/Format: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Sequence Positions: Full length
• Sequence Length: 2739

• Applicable Applications for CYCE2 recombinant protein: Western Blot (WB)
• Species: Human
• Tag: GST tag
• Preparation and Storage: Store product at -70 degree C.; For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.; For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Testing Data

Related Product Information for CYCE2 recombinant protein

Description: Recombinant full-length human CyclinE2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: CCNE2 is belongs to the cyclin family, Cyclin E subfamily. It is encoded by a separate gene from Cyclin E1, but shows substantial amino acid sequence similarity. Like Cyclin E1, it binds and activates the cyclin-dependent kinase 2 (Cdk2), which in turn phosphorylates proteins involved in cell cycle progression such as pRB, p107, NPM1, MCMs, thereby driving cell proliferation. A defect in spermatogenesis is the only phenotype observed in cyclin E2-deficient mice, suggesting its role in male fertility.

Product Categories/Family for CYCE2 recombinant protein

Signaling Proteins; Cell Cycle Proteins

References

1. Geng Y, et al. (2003) Cyclin E ablation in the mouse. Cell 114: 431 -443. 2. Hwang HC, et al. (2005) Cyclin E in normal and neoplastic cell cycles. Oncogene 24: 2776 -2786. 3. Odajima J, et al. (2016). Proteomic Landscape of Tissue-Specific Cyclin E Functions in Vivo. PLoS Genet. 12 (11):e1006429.

NCBI and Uniprot Product Information

• NCBI GI #: 325910865
• NCBI GeneID: 9134
• NCBI Accession #: NM_057749.2
• NCBI GenBank Nucleotide #: NM_057749
• UniProt Accession #: O96020; O95439
• Molecular Weight: 72 kDa
• NCBI Official Full Name: Homo sapiens cyclin E2 (CCNE2), mRNA
• NCBI Official Synonym Full Names: cyclin E2
• NCBI Official Symbol: CCNE2
• NCBI Official Synonym Symbols: CYCE2
• NCBI Protein Information: G1/S-specific cyclin-E2
• UniProt Protein Name: G1/S-specific cyclin-E2
• UniProt Gene Name: CCNE2

NCBI Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2. This cyclin has been shown to specifically interact with CIP/KIP family of CDK inhibitors, and plays a role in cell cycle G1/S transition. The expression of this gene peaks at the G1-S phase and exhibits a pattern of tissue specificity distinct from that of cyclin E1. A significantly increased expression level of this gene was observed in tumor-derived cells. [provided by RefSeq, Jul 2008]

Uniprot Description

CCNE2: Essential for the control of the cell cycle at the late G1 and early S phase. Interacts with the CDK2 (in vivo) and CDK3 (in vitro) protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Activated by papilloma viral oncoproteins E6 and E7 which bind to and inactivate p53 and Rb, respectively. According to PubMed:9858585, highest levels of expression in adult testis, thymus and brain. Lower levels in placenta, spleen and colon. Consistently elevated levels in tumor- derived cells compared to non-transformed proliferating cells. According to PubMed:9840927: low levels in thymus, prostate, brain, skeletal muscle, and kidney. Elevated levels in lung. According to PubMed:9840943 highly expressed in testis, placenta, thymus and brain. In a lesser extent in small intestine and colon. Belongs to the cyclin family. Cyclin E subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Activator; Cell cycle regulation

Chromosomal Location of Human Ortholog: 8q22.1

Cellular Component: cytosol; nucleoplasm

Molecular Function: protein binding

Biological Process: cell cycle checkpoint; G1/S transition of mitotic cell cycle; regulation of cyclin-dependent protein kinase activity