CDKN3 recombinant protein

CDKN3 recombinant protein

Cat. Num. AAA515137

• Gene Names: CDKN3; KAP; CDI1; CIP2; KAP1
• Applications: Western Blot
• Synonyms: CDKN3; CDKN3 recombinant protein; CDI1; CIP2; FLJ25787; KAP; KAP1; MGC70625

• Host: Sf9 insect cells
• Form/Format: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Sequence Length: 906

• Applicable Applications for CDKN3 recombinant protein: Western Blot (WB)
• Type: Recombinant Fusion Protein
• Species: Human
• Tag Information: GST tag
• Expression System: Sf9 insect cells using baculovirus
• Source Note: Recombinant full-length human CDKN3 was expressed by baculovirus in Sf9 insect cells
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

Related Product Information for CDKN3 recombinant protein

Recombinant full-length human CDKN3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Scientific Background: CDKN3 is a member of the dual specificity protein phosphatase family that acts as a cyclin-dependent kinase inhibitor (1). CDKN3 has been shown to interact with and dephosphorylate specifically the CDK2 kinase thereby preventing it's activation. CDKN3 has been reported to be deleted, mutated, or overexpressed in several kinds of cancers. Increased expression of CDKN3 leads to increased levels of kinase-associated phosphatase activity that inhibits the G(1)/S transition of the cell cycle by dephosphorylating the cyclin-dependent kinases (2).

Product Categories/Family for CDKN3 recombinant protein

Cell Cycle Proteins; Signaling Proteins - Cell Cycle Proteins

References

1. Niculescu, M.D. et al : Choline availability modulates human neuroblastoma cell proliferation and alters the methylation of the promoter region of the cyclin-dependent kinase inhibitor 3 gene. J Neurochem. 2004, 89(5):1252-9.
2.Yeh, C.T. et al: Abolishment of the interaction between cyclin-dependent kinase 2 and Cdk-associated protein phosphatase by a truncated KAP mutant. Biochem. Biophys. Res. Commun. 2003; 305(2):311-4

NCBI and Uniprot Product Information

• NCBI GI #: 195927023
• NCBI GeneID: 1033
• NCBI Accession #: NP_005183.2
• NCBI GenBank Nucleotide #: NM_005192
• UniProt Accession #: Q16667; Q53ZU6; Q5U0M4; Q6P1N8; Q99585; Q9BPW7; Q9BY36; Q9C042; Q9C046; Q9C047; Q9C049; Q9C051
• Molecular Weight: ~51 kDa
• NCBI Official Full Name: Homo sapiens cyclin-dependent kinase inhibitor 3 (CDKN3), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: cyclin-dependent kinase inhibitor 3
• NCBI Official Symbol: CDKN3
• NCBI Official Synonym Symbols: KAP; CDI1; CIP2; KAP1
• NCBI Protein Information: cyclin-dependent kinase inhibitor 3; kinase associated phosphatase; kinase-associated phosphatase; Cdk-associated protein phosphatase; cyclin-dependent kinase interactor 1; CDK2-associated dual specificity phosphatase; CDK2-associated dual-specificity phosphatase; cyclin-dependent kinase interacting protein 2; cyclin-dependent kinase-interacting protein 2
• UniProt Protein Name: Cyclin-dependent kinase inhibitor 3
• Protein Family: Cyclin-dependent kinase inhibitor
• UniProt Gene Name: CDKN3
• UniProt Synonym Gene Names: CDI1; CIP2; KAP
• UniProt Entry Name: CDKN3_HUMAN

NCBI Description

The protein encoded by this gene belongs to the dual specificity protein phosphatase family. It was identified as a cyclin-dependent kinase inhibitor, and has been shown to interact with, and dephosphorylate CDK2 kinase, thus prevent the activation of CDK2 kinase. This gene was reported to be deleted, mutated, or overexpressed in several kinds of cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

Function: May play a role in cell cycle regulation. Dual specificity phosphatase active toward substrates containing either phosphotyrosine or phosphoserine residues. Dephosphorylates CDK2 at 'Thr-160' in a cyclin-dependent manner. Ref.1 Ref.2 Ref.12

Catalytic activity: Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.A phosphoprotein + H2O = a protein + phosphate.

Subunit structure: Interacts with cyclin-dependent kinases such as CDK1, CDK2 and CDK3. Does not interact with CDK4. Interacts (via C-terminus) with phosphorylated CDK2 (via C-terminal helix). Interacts with MS4A3 (via C-terminus); the interaction enhances CDKN3 enzymatic activity. Ref.1 Ref.2 Ref.4 Ref.14 Ref.15

Subcellular location: Cytoplasm › perinuclear region Ref.13.

Induction: Up-regulated in breast and prostate cancer cells. Ref.13

Involvement in disease: Hepatocellular carcinoma (HCC) [MIM:114550]: A primary malignant neoplasm of epithelial liver cells. The major risk factors for HCC are chronic hepatitis B virus (HBV) infection, chronic hepatitis C virus (HCV) infection, prolonged dietary aflatoxin exposure, alcoholic cirrhosis, and cirrhosis due to other causes.Note: The gene represented in this entry may be involved in disease pathogenesis. Ref.3

Sequence similarities: Belongs to the protein-tyrosine phosphatase family.