CDK3/CyclinE1 Recombinant Protein | CDK3 recombinant protein

CDK3/CyclinE1, Active

Cat. Num. AAA515476

• Applications: Western Blot
• Synonyms: CDK3/CyclinE1; Active; CDK3/CyclinE2; CDK3 recombinant protein

• Host: Sf9 insect cells using baculovirus
• Form/Format: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
• Sequence Positions: Full Length
• Sequence Length: 1584

• Applicable Applications for CDK3 recombinant protein: Kinase Assay, Western Blot (WB)
• Species: Human
• Tag: GST tag
• Preparation and Storage: Store product at -70 degree C.; For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.; For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Testing Data

Testing Data #2

Related Product Information for CDK3 recombinant protein

Description: Recombinant full-length human CDK3 and Cyclin E1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.
Scientific Background: CDK3/CyclinE1is a member of the cyclin-dependent protein kinase family and promotes entry into S phase, in part by activating members of the E2F family of transcription factors. CDK3 can also associate with cyclin C and phosphorylates the retinoblastoma 1 protein to promote exit from G0. The CDK3 gene has been mapped to chromosomal location of 17q22-qter, telomeric to the BRCA1 gene by somatic cell hybrids analysis (1). The presence of a single point mutation in the CDK3 gene from several Mus musculus strains commonly used in the laboratory has been reported (2).

Product Categories/Family for CDK3 recombinant protein

Enzymes; Active Kinases

References

1. Bullrich, F. et al: Chromosomal mapping of members of the cdc2 family of protein kinases, cdk3, cdk6, PISSLRE, and PITALRE, and a cdk inhibitor, p27-Kip1, to regions involved in human cancer. Cancer Res. 55: 1199-1205, 1995. 2. Ye, X. et al: A premature-termination mutation in the Mus musculus cyclin-dependent kinase 3 gene. Proc. Nat. Acad. Sci. 98: 1682-1686, 2001.

NCBI and Uniprot Product Information

• NCBI GI #: 164519034
• NCBI GeneID: 1018
• NCBI Accession #: NM_001258.2
• NCBI GenBank Nucleotide #: NM_001258
• Molecular Weight: ~60 kDa; ~73 kDa
• NCBI Official Full Name: Homo sapiens cyclin dependent kinase 3 (CDK3), mRNA
• NCBI Official Synonym Full Names: cyclin dependent kinase 3
• NCBI Official Symbol: CDK3
• NCBI Protein Information: cyclin-dependent kinase 3
• UniProt Protein Name: Cyclin-dependent kinase 3
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: CDK3
• UniProt Synonym Gene Names: CDKN3

NCBI Description

This gene encodes a member of the cyclin-dependent protein kinase family. The protein promotes entry into S phase, in part by activating members of the E2F family of transcription factors. The protein also associates with cyclin C and phosphorylates the retinoblastoma 1 protein to promote exit from G0. [provided by RefSeq, Jul 2008]

Uniprot Description

CDK3: a protein kinase of the CDK family. May be involved in cell cycle control. Can phosphorylate histone H1.

Protein type: CDK family; CDK/CDK1 subfamily; CDK1 subfamily; CMGC group; Cell cycle regulation; EC 2.7.11.22; Kinase, protein; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: cyclin-dependent protein kinase holoenzyme complex; cytoplasm; nucleus

Molecular Function: cyclin binding; cyclin-dependent protein kinase activity; protein binding

Biological Process: cell proliferation; G0 to G1 transition; G1/S transition of mitotic cell cycle; multicellular organismal development; positive regulation of cell proliferation; protein amino acid phosphorylation; regulation of G2/M transition of mitotic cell cycle; regulation of gene expression; response to DNA damage stimulus; response to organic substance; signal transduction