Western Blot (WB)
Rabbit beta-Actin Polyclonal Antibody | anti-beta-actin antibody
Anti-beta-Actin Antibody
Applications
Western Blot, Immunoprecipitation
Synonyms
beta-Actin; Polyclonal Antibody; Anti-beta-Actin Antibody; anti-beta-actin antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognize beta-actin from Human and Mouse. Other species not tested yet.
Form/Format
Liquid
Concentration
100ug/100ul (varies by lot)
Sequence Length
124
Applicable Applications for anti-beta-actin antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use.
Suggested starting dilutions are as follows:
Western Blot: 1:500
IP: 5 mcg/mg lysate.
Suggested starting dilutions are as follows:
Western Blot: 1:500
IP: 5 mcg/mg lysate.
Storage Buffer
PBS, pH 7.4 with 0.02% Sodium Azide
Immunogen
Syntheitic peptide at the N-terminus of Beta-Actin. The N-terminus of Beta-Actin is highly conserved with Gamma-Actin.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Western Blot (WB)
Western Blot (WB)
References
Horakova, AH et al. "The macrosatellite DXZ4 mediates CTCF-dependent long-range intrachromosomal interactions on the human inactive X chromosome." Hum Mol Genet 21 (20):4367-4377 (2012). DOI: 22791747. Application: Western Blot.
Kim, BH et al. "Hairless plays a role in formation of inner root sheath via regulation of Dlx3 gene." J Biol Chem 287 (20):16681-16688 (2012). DOI: 10.1074/jbc.M111.320770. Application: Western Blot.
Chaiyakul, M et al. "Cytotoxicity of ORF3 proteins from a nonpathogenic and a pathogenic porcine circovirus." J Virol 84 (21):11440-11447 (2010). DOI: 10.1128/JVI.01030-10. Application: Western Blot.
Sanz-Clemente, A et al. "Casein kinase 2 regulates the NR2 subunit composition of synaptic NMDA receptors." Neuron 67:984-96 (2010). PubMed: 20869595.
Kim, BH et al. "Hairless plays a role in formation of inner root sheath via regulation of Dlx3 gene." J Biol Chem 287 (20):16681-16688 (2012). DOI: 10.1074/jbc.M111.320770. Application: Western Blot.
Chaiyakul, M et al. "Cytotoxicity of ORF3 proteins from a nonpathogenic and a pathogenic porcine circovirus." J Virol 84 (21):11440-11447 (2010). DOI: 10.1128/JVI.01030-10. Application: Western Blot.
Sanz-Clemente, A et al. "Casein kinase 2 regulates the NR2 subunit composition of synaptic NMDA receptors." Neuron 67:984-96 (2010). PubMed: 20869595.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
13,850 Da
NCBI Official Full Name
beta-actin, partial
UniProt Protein Name
Beta-actin
Protein Family
UniProt Gene Name
beta-actin
UniProt Entry Name
K4NQ23_9EURO
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Product Notes
The beta-Actin beta-actin (Catalog #AAA416880) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's beta-Actin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows: Western Blot: 1:500 IP: 5 mcg/mg lysate. Researchers should empirically determine the suitability of the beta-Actin beta-actin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta-Actin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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