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SDS-PAGE

CyclinE1 Recombinant Protein | CCNE1 recombinant protein

CyclinE1 recombinant protein

Gene Names
CCNE1; CCNE
Applications
Western Blot
Synonyms
CyclinE1; CyclinE1 recombinant protein; CCNE1; CCNE; CCNE1 recombinant protein
Ordering
For Research Use Only!
Host
Sf9 insect cells
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Sequence Length
2021
Applicable Applications for CCNE1 recombinant protein
Kinase Assay, Western Blot (WB)
Type
Recombinant Fusion Protein
Species
Human
Tag Information
GST tag
Expression System
Sf9 insect cells using baculovirus
Source Note
Recombinant full-length human CyclinE1 was expressed by baculovirus in Sf9 insect cells
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

SDS-PAGE
Related Product Information for CCNE1 recombinant protein
Recombinant full-length human CyclinE1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Scientific Background: Cyclin E1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle that functions as regulators of CDK kinases. Cyclin E1 activity is required for centrosome duplication during S phase and this mechanism could coordinate centrosome reproduction with cycles of DNA synthesis and mitosis (1). The downregulation of cyclin E-CDK2 kinase activity following the G1/S-phase transition that is necessary for the maintenance of karyotypic stability. Cyclin E also has a modular centrosomal-targeting domain which is essential for promoting S phase entry in a Cdk2-independent manner (2).
Product Categories/Family for CCNE1 recombinant protein
References
1. Hinchcliffe, E. H. et.al: Requirement of Cdk2-cyclin E activity for repeated centrosome reproduction in Xenopus egg extracts. Science 283: 851-854, 1999.
2. Matsumoto, Y.et.al: A centrosomal localization signal in cyclin E required for Cdk2-independent S phase entry. Science 306: 885-888, 2004.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
898
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~73kDa
NCBI Official Full Name
Homo sapiens cyclin E1 (CCNE1), mRNA
NCBI Official Synonym Full Names
cyclin E1
NCBI Official Symbol
CCNE1
NCBI Official Synonym Symbols
CCNE
NCBI Protein Information
G1/S-specific cyclin-E1; cyclin Es; cyclin Et
UniProt Protein Name
G1/S-specific cyclin-E1
UniProt Gene Name
CCNE1
UniProt Synonym Gene Names
CCNE
UniProt Entry Name
CCNE1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Essential for the control of the cell cycle at the G1/S (start) transition. Ref.3

Subunit structure: Interacts with a member of the CDK2/CDK protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Found in a complex with CDK2, CABLES1 and CCNA1

By similarity. Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts directly with UHRF2; the interaction ubiquitinates CCNE1 and appears to occur independently of CCNE1 phosphorylation. Ref.13 Ref.17

Subcellular location: Nucleus Ref.3.

Tissue specificity: Highly expressed in testis and placenta. Low levels in bronchial epithelial cells. Ref.11

Post-translational modification: Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7.Ubiquitinated by UHRF2; appears to occur independently of phosphorylation. Ref.17

Sequence similarities: Belongs to the cyclin family. Cyclin E subfamily.

Research Articles on CCNE1

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Product Notes

The CCNE1 ccne1 (Catalog #AAA515099) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is GST tag!!Expression System||Sf9 insect cells using baculovirus!!Source Note||Recombinant full-length human CyclinE1 was expressed by baculovirus in Sf9 insect cells. AAA Biotech's CyclinE1 can be used in a range of immunoassay formats including, but not limited to, Kinase Assay, Western Blot (WB). Researchers should empirically determine the suitability of the CCNE1 ccne1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CyclinE1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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