MIP-3 alpha (CCL20) Recombinant Protein | MIP-3A recombinant protein

Human MIP-3 alpha (CCL20) Recombinant

Cat. Num. AAA553195

• Purity: >98%, as determined by SDS-PAGE and HPLC
• Synonyms: MIP-3 alpha (CCL20); Human MIP-3 alpha (CCL20) Recombinant; Human MIP-3A (CCL20) Recombinant; ccl-20 ; CCL20; mip3a; mip-3a; SCYA20 ; LARC; MIP-3A recombinant protein

• Host: E Coli
• Purity/Purification: >98%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant CCL20 was lyophilized from a 0.2 um filtered 20mM PB,100mM NaCl solution pH 7.5.
• Sequence: MCCTKSLLLA ALMSVLLLHL CGESEAASNF DCCLGYTDRI LHPKFIVGFT RQLANEGCDI NAIIFHTKKK LSVCANPKQT WVKYIVRLLS KKVKNM
• Sequence Length: 95

• Domain: IL8
• Host Note: Optimized DNA sequence encoding Human MIP-3 alpha (CCL20) mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Human CCL20, generated by the proteolytic removal of the signal peptide; and propeptide and has a calculated molecular mass of approximately 8 kDa.; ; Recombinant CCL-20 is a disulfide-linked monomeric protein consisting of 71 amino acid residue subunits and migrates as an approximately 8 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for MIP-3A recombinant protein

Macrophage inflammatory protein (MIP) -3/CCL20, also known as liver and activation-regulated chemokine (LARC) or Exodus, is a member of the CC chemokine subfamily initially noted to be expressed in human liver, lung, appendix, and tonsillar crypts. MIP-3 is selectively chemotactic for CD34+ bone marrow cell-derived immature DCs and CD45RO+ memory T cells that express the cognate receptor CCR6. MIP-3 produced at sites of inflammation may chemoattract CCR6-expressing immature DCs to the subepithelial region of mucosal surfaces.

Product Categories/Family for MIP-3A recombinant protein

Cytokines

References

• proinflammatory cytokines synergistically enhance the production of chemokine ligand 20 (CCL20) from rheumatoid fibroblast-like synovial cells in vitro and serum CCL20 is reduced in vivo by biologic disease-modifying antirheumatic drugs .J Rheumatol, Nov 2009; 36: 2397 - 2402.
• interplay between human adipocytes and t lymphocytes in obesity: CCL20 as an adipochemokine and t lymphocytes as lipogenic modulators .Arterioscler Thromb Vasc Biol, Oct 2009; 29: 1608 - 1614.
• plc, p38/mapk, and nf b-mediated induction of mip-3/CCL20 by porphyromonas gingivalis .Innate Immunity, Aug 2009; 10.1177/1753425909339237.
• serum amyloid a protein stimulates CCL20 production in rheumatoid synoviocytes .Rheumatology, Jul 2009; 48: 741 - 747.
• modulation of expression of innate immunity markers cxcl5/ena-78 and CCL20/mip3 by protease activated receptors (pars) in human gingival epithelial cells .Innate Immunity, Jun 2009; 10.1177/1753425909339233.