Mouse anti-Human CCL20 / MIP-3-Alpha Monoclonal Antibody | anti-CCL20 antibody

Mouse Monoclonal (IgG1,k) to Human CCL20 / MIP-3-Alpha

Cat. Num. AAA245814

• Gene Names: CCL20; CKb4; LARC; ST38; MIP3A; Exodus; MIP-3a; SCYA20; MIP-3-alpha
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: CCL20 / MIP-3-Alpha; Monoclonal Antibody; Mouse Monoclonal (IgG1; k) to Human CCL20 / MIP-3-Alpha; Anti-CCL20 / MIP-3-Alpha Antibody IHC-plus; CCL20; Beta chemokine exodus-1; Beta-chemokine exodus-1; CKb4; Exodus-1; MIP-3a; MIP-3-alpha; LARC; ST38; SCYA20; C-C motif chemokine 20; CC chemokine LARC; Mip-3alpha; MIP3A; Small-inducible cytokine A20; Human CCL20; MIP-3-Alpha; anti-CCL20 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Specificity: Human MIP-3alpha
• Purity/Purification: Protein A Purified
• Form/Format: Lyophilized from PBS. Sourced in TCS.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 96

• Applicable Applications for anti-CCL20 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (2 - 4 ug/ml), IHC-P (10 ug/ml), WB (0.2 - 0.4 ug/ml); Usage: Immunohistochemistry: in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 10 ug/ml is recommended. ELISA: In a sandwich...
• Target Species: Human
• Resuspension Liquid Description: 500 ul sterile water
• Immunogen Description: E.coli derived recombinant Human MIP-3alpha.
• Immunogen Type: Cell extract
• Immunogen: CCL20 / MIP-3-Alpha antibody was raised against e.coli derived recombinant Human MIP-3alpha.
• Preparation and Storage: Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8 degree C. Aliquot and freeze at -20 degree C for long term storage. Avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-CCL20 antibody IHC of human spleen, lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Related Product Information for anti-CCL20 antibody

Chemokines are a family of related proteins that regulate leukocyte migration (including into tissues) and activation in the face of inflammatory stimulus. CCL20/LARC/EXODUS/MIP-3 has been shown to be highly expressed in liver, and preferentially in lymphocytes and monocytes where its expression is upregulated by mediators of inflammation.

Product Categories/Family for anti-CCL20 antibody

Family: Intercrine; Subfamily: Intercrine beta

NCBI and Uniprot Product Information

• NCBI GI #: 4759076
• NCBI GeneID: 6364
• NCBI Accession #: NP_004582.1
• NCBI GenBank Nucleotide #: NM_004591.2
• UniProt Accession #: P78556; Q53S51; Q99664
• Molecular Weight: 10,691 Da
• NCBI Official Full Name: C-C motif chemokine 20 isoform 1
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 20
• NCBI Official Symbol: CCL20
• NCBI Official Synonym Symbols: CKb4; LARC; ST38; MIP3A; Exodus; MIP-3a; SCYA20; MIP-3-alpha
• NCBI Protein Information: C-C motif chemokine 20; CC chemokine LARC; beta chemokine exodus-1; small-inducible cytokine A20; macrophage inflammatory protein 3 alpha; liver and activation-regulated chemokine; small inducible cytokine subfamily A (Cys-Cys), member 20
• UniProt Protein Name: C-C motif chemokine 20
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL20
• UniProt Synonym Gene Names: LARC; MIP3A; SCYA20; MIP-3-alpha
• UniProt Entry Name: CCL20_HUMAN

NCBI Description

This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene displays chemotactic activity for lymphocytes and can repress proliferation of myeloid progenitors. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

CCL20: Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213. Belongs to the intercrine beta (chemokine CC) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Chemokine; Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 2q36.3

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; chemokine activity

Biological Process: cell-cell signaling; wound healing; defense response to bacterium; immune response; inflammatory response; chemotaxis; signal transduction; positive regulation of interleukin-1 alpha biosynthetic process; positive regulation of nitric-oxide synthase biosynthetic process