Monkey Macrophage Inflammatory Protein-3alpha (MIP-3alpha/CCL20) ELISA Kit | MIP-3alpha/CCL20 elisa kit

Monkey Macrophage Inflammatory Protein-3alpha, MIP-3alpha/CCL20 ELISA Kit

Cat. Num. AAA932985

• Gene Names: CCL20; CKb4; LARC; ST38; MIP3A; MIP-3a; SCYA20; MIP-3-alpha
• Reactivity: Monkey
• Synonyms: Macrophage Inflammatory Protein-3alpha (MIP-3alpha/CCL20); Monkey Macrophage Inflammatory Protein-3alpha; MIP-3alpha/CCL20 ELISA Kit; Monkey Macrophage Inflammatory Protein-3alpha (MIP-3alpha/CCL20) ELISA Kit; CKb4; LARC; MIP-3a; MIP3A; SCYA20; ST38; exodus-1; macrophage inflammatory protein 3 alpha; small inducible cytokine subfamily A (Cys-Cys); member 20; MIP-3alpha/CCL20 elisa kit

• Reactivity: Monkey
• Specificity: This assay has high sensitivity and excellent specificity for detection of monkey MIP-3alpha/CCL20. No significant cross-reactivity or interference between monkey MIP-3alpha/CCL20 and analogues was observed.
• Sequence Length: 95

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 31.25 pg/ml -2000 pg/ml.
• Sensitivity: The minimum detectable dose of monkey MIP-3alpha/CCL20 is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MIP-3alpha/CCL20 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MIP-3alpha/CCL20 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MIP-3alpha/CCL20 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MIP-3alpha/CCL20 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MIP-3alpha/CCL20 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 194239688
• NCBI GeneID: 6364
• NCBI Accession #: NP_001123518.1
• NCBI GenBank Nucleotide #: NM_001130046.1
• UniProt Accession #: P78556; Q53S51; Q99664
• Molecular Weight: 10,762 Da
• NCBI Official Full Name: C-C motif chemokine 20 isoform 2
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 20
• NCBI Official Symbol: CCL20
• NCBI Official Synonym Symbols: CKb4; LARC; ST38; MIP3A; MIP-3a; SCYA20; MIP-3-alpha
• NCBI Protein Information: C-C motif chemokine 20; CC chemokine LARC; beta chemokine exodus-1; small-inducible cytokine A20; macrophage inflammatory protein 3 alpha; liver and activation-regulated chemokine; small inducible cytokine subfamily A (Cys-Cys), member 20
• UniProt Protein Name: C-C motif chemokine 20
• UniProt Gene Name: CCL20
• UniProt Synonym Gene Names: LARC; MIP3A; SCYA20; MIP-3-alpha
• UniProt Entry Name: CCL20_HUMAN

NCBI Description

This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene displays chemotactic activity for lymphocytes and can repress proliferation of myeloid progenitors. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

CCL20: Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213. Belongs to the intercrine beta (chemokine CC) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Chemokine; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q36.3

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; chemokine activity

Biological Process: cell-cell signaling; wound healing; defense response to bacterium; immune response; positive regulation of interleukin-1 alpha biosynthetic process; signal transduction; inflammatory response; chemotaxis; positive regulation of nitric-oxide synthase biosynthetic process