Goat CCL20 / MIP-3-Alpha Polyclonal Antibody | anti-CCL20 antibody

Goat Polyclonal to Human CCL20 / MIP-3-Alpha

Cat. Num. AAA247211

• Gene Names: CCL20; CKb4; LARC; ST38; MIP3A; Exodus; MIP-3a; SCYA20; MIP-3-alpha
• Reactivity: Gorilla, Human; Predicted Reactivity: Gibbon, Monkey (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: CCL20 / MIP-3-Alpha; Polyclonal Antibody; Goat Polyclonal to Human CCL20 / MIP-3-Alpha; Anti-CCL20 / MIP-3-Alpha Antibody (Internal) IHC-plus; CCL20; Beta chemokine exodus-1; Beta-chemokine exodus-1; CKb4; Exodus-1; MIP-3a; MIP-3-alpha; LARC; ST38; SCYA20; C-C motif chemokine 20; CC chemokine LARC; Mip-3alpha; MIP3A; Small-inducible cytokine A20; Human CCL20; MIP-3-Alpha; anti-CCL20 antibody

• Host: Goat
• Reactivity: Gorilla, Human; Predicted Reactivity: Gibbon, Monkey (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human CCL20. This antibody is expected to recognize both isoforms (NP_004582.1; NP_001123518.1).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 96

• Applicable Applications for anti-CCL20 antibody: Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
• Application Notes: ELISA (1:16000), IHC-P (6.12 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Preliminary experiments in Human Peripheral Blood Mononucleocytes lysates gave no specific signal but low background (at antibody concentration up to 2 ug/ml).
• Target Species: Human
• Immunogen Description: Synthetic peptide CLGYTDRILHPK from an internal region of human CCL20 (NP_004582.1; NP_001123518.1). Percent identity by BLAST analysis: Human, Gorilla (100%); Gibbon, Monkey (92%).
• Immunogen Type: Synthetic peptide
• Immunogen: CCL20 / MIP-3-Alpha antibody was raised against synthetic peptide CLGYTDRILHPK from an internal region of human CCL20 (NP_004582.1; NP_001123518.1). Percent identity by BLAST analysis: Human, Gorilla (100%); Gibbon, Monkey (92%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-CCL20 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 6.125 ug/ml.)

Related Product Information for anti-CCL20 antibody

Chemokines are a family of related proteins that regulate leukocyte migration (including into tissues) and activation in the face of inflammatory stimulus. CCL20/LARC/EXODUS/MIP-3 has been shown to be highly expressed in liver, and preferentially in lymphocytes and monocytes where its expression is upregulated by mediators of inflammation.

Product Categories/Family for anti-CCL20 antibody

Family: Intercrine; Subfamily: Intercrine beta

NCBI and Uniprot Product Information

• NCBI GI #: 4759076
• NCBI GeneID: 6364
• NCBI Accession #: NP_004582.1
• NCBI GenBank Nucleotide #: NM_004591.2
• UniProt Accession #: P78556; Q53S51; Q99664
• Molecular Weight: 10,691 Da
• NCBI Official Full Name: C-C motif chemokine 20 isoform 1
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 20
• NCBI Official Symbol: CCL20
• NCBI Official Synonym Symbols: CKb4; LARC; ST38; MIP3A; Exodus; MIP-3a; SCYA20; MIP-3-alpha
• NCBI Protein Information: C-C motif chemokine 20; CC chemokine LARC; beta chemokine exodus-1; small-inducible cytokine A20; macrophage inflammatory protein 3 alpha; liver and activation-regulated chemokine; small inducible cytokine subfamily A (Cys-Cys), member 20
• UniProt Protein Name: C-C motif chemokine 20
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL20
• UniProt Synonym Gene Names: LARC; MIP3A; SCYA20; MIP-3-alpha
• UniProt Entry Name: CCL20_HUMAN

NCBI Description

This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene displays chemotactic activity for lymphocytes and can repress proliferation of myeloid progenitors. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

CCL20: Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213. Belongs to the intercrine beta (chemokine CC) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Motility/polarity/chemotaxis; Chemokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2q36.3

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; chemokine activity

Biological Process: cell-cell signaling; wound healing; defense response to bacterium; immune response; signal transduction; chemotaxis; positive regulation of interleukin-1 alpha biosynthetic process; inflammatory response; positive regulation of nitric-oxide synthase biosynthetic process