SDS-PAGE
Stathmin Recombinant Protein | STMN1 recombinant protein
Stathmin recombinant protein
SDS-PAGE
SDS-PAGE
Scientific Background: Stathmin is a ubiquitous, highly conserved protein present in the cytoplasm in a variety of unphosphorylated and phosphorylated forms. Stathmin functions as an intracellular relay integrating regulatory signals of the cellular environment (1). Stathmin is involved in the regulation of the microtubule filament system. Stathmin binds tubulin in a ternary complex, with 2 tubulins for every stathmin and this complex interferes with microtubule dynamics in vitro and in vivo. Overexpression of several stathmin mutants suggest that stathmin induces depolymerization of interphase and mitotic microtubules in its unphosphorylated state, but is inactivated by phosphorylation in mitosis (2).
2. Gavet, O. et al: The stathmin phosphoprotein family: intracellular localization and effects on the microtubule network. J. Cell Sci. 111: 3333-3346, 1998
NCBI and Uniprot Product Information
NCBI Description
This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]
Uniprot Description
Function: Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear
By similarity.
Subunit structure: Binds to two alpha/beta-tubulin heterodimers. Interacts with KIST. Ref.15 Ref.16
Subcellular location: Cytoplasm › cytoskeleton.
Tissue specificity: Ubiquitous. Expression is strongest in fetal and adult brain, spinal cord, and cerebellum, followed by thymus, bone marrow, testis, and fetal liver. Expression is intermediate in colon, ovary, placenta, uterus, and trachea, and is readily detected at substantially lower levels in all other tissues examined. Lowest expression is found in adult liver. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, non-leukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia. Ref.3 Ref.18
Induction: Down-regulated in response to enterovirus 71 (EV71) infection (at protein level). Ref.20
Post-translational modification: Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization
By similarity. Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity. Ref.11 Ref.12 Ref.13 Ref.14 Ref.17
Sequence similarities: Belongs to the stathmin family.Contains 1 SLD (stathmin-like) domain.
Research Articles on STMN1
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Product Notes
The STMN1 stmn1 (Catalog #AAA515238) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His tag!!Expression System||E.coli!!Source Note||Recombinant full-length human Stathmin protein was expressed in E. coli cells. AAA Biotech's Stathmin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the STMN1 stmn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Stathmin, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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