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Testing Data

Nitrotyrosine Protein

Nitrotyrosine Protein Standard

Purity
95 % as assessed by vis
Synonyms
Nitrotyrosine; Nitrotyrosine Protein Standard; Nitrotyrosine Immunoblot Positive Control; Nitrotyrosine protein
Ordering
For Research Use Only!
Host
Carbonic anhydrase from bovine erythrocytes
Purity/Purification
95 % as assessed by vis
Concentration
200 mug/mL total protein using the Bradford protein assay with BSA as a standard (varies by lot)
Storage Buffer
Phosphate buffered saline (PBS), pH 7.4 containing 20% Glycerol.
Preparation and Storage
At -20 degree C AVOID MULTIPLE FREEZE-THAW CYCLES.

Testing Data

Testing Data
Related Product Information for Nitrotyrosine protein
Description: This is a positive control to be used in conjunction with Anti- Nitrotyrosine (NT50) when performing immunoblots. It contains nitrated carbonic anhydrase (Mr=29kDa) in PBS.
References
Ye YZ, Strong M, Huang ZQ, Beckman S. Methods Enzymol. 269, 201 (1996).Figure 1. Western blot using nitrotyrosine immunoblot positive control on nitrocellulose with OBR's nitrotyrosine antibody (prod no. NS50) at a 1:1,000 dilution. Lane 1, 2.44mug; Lane 2, 9.75ng nitrotyrosine immunoblot positive control

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Product Notes

The Nitrotyrosine (Catalog #AAA480652) is a Protein produced from Carbonic anhydrase from bovine erythrocytes and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Nitrotyrosine, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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