Carbonic Anhydrase 4/CA4 Active Protein | CA4 active protein

Human CellExp Carbonic Anhydrase 4 / CA4, human recombinant

Cat. Num. AAA844352

• Gene Names: CA4; CAIV; Car4; RP17
• Applications: SDS-Page
• Purity: >=92%
• Synonyms: Carbonic Anhydrase 4/CA4; Human CellExp Carbonic Anhydrase 4 / CA4; human recombinant; CA4; Carbonic anhydrase 4; CA4 active protein

• Host: HEK293 cells
• Purity/Purification: >=92%
• Form/Format: Lyophilized from 0.22 mum filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.; Appearance: Lyophilized
• Sequence Length: 312

• Applicable Applications for CA4 active protein: SDS-PAGE
• Solubility/Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
• Endotoxin Level: <1 EU/mug by LAL method
• Biological Activity: Measured by its esterase activity. The specific activity is >2 pmol/min/ug.
• Results: Measured by its esterase activity. The specific activity is >2 pmol/min/ug.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for CA4 active protein

Background: Carbonic anhydrase 4 (CA4) is also known as Carbonate dehydratase IV, Carbonic anhydrase IV, which belongs to the alpha-carbonic anhydrase family. CA4 may stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid. The enzyme regulation is activated by histamine, L-adrenaline, D-phenylalanine, L- and D-histidine. CA4 can interact with SLC4A4.

Product Categories/Family for CA4 active protein

Proteins and Enzymes; Human cell expressed recombinant proteinsProteins and Enzymes; Proteins and Enzymes (A-Z) Proteins and Enzymes; Other Proteins & EnzymesDiabetes; Obesity & Metabolic Syndrome; Lipids Metabolism; Proteins/Enzymes; Other (NEW)

NCBI and Uniprot Product Information

• NCBI GI #: 4502519
• NCBI GeneID: 762
• NCBI Accession #: NP_000708.1
• NCBI GenBank Nucleotide #: NM_000717.3
• UniProt Accession #: P22748; Q6FHI7; B4DQA4
• Molecular Weight: This protein is fused with polyhistidine tag at the C-terminus, and has a calculated MW of 31 kDa. The predicted N-terminus is Ala 19. DTT-reduced Protein migrates as 33 kDa in SDS-PAGE.
• NCBI Official Full Name: carbonic anhydrase 4
• NCBI Official Synonym Full Names: carbonic anhydrase IV
• NCBI Official Symbol: CA4
• NCBI Official Synonym Symbols: CAIV; Car4; RP17
• NCBI Protein Information: carbonic anhydrase 4
• UniProt Protein Name: Carbonic anhydrase 4
• Protein Family: Carbonic anhydrase
• UniProt Gene Name: CA4
• UniProt Synonym Gene Names: CA-IV
• UniProt Entry Name: CAH4_HUMAN

NCBI Description

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This gene encodes a glycosylphosphatidyl-inositol-anchored membrane isozyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and proximal renal tubules. Its exact function is not known; however, it may have a role in inherited renal abnormalities of bicarbonate transport. [provided by RefSeq, Jul 2008]

Uniprot Description

CA4: Reversible hydration of carbon dioxide. May stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid. Defects in CA4 are the cause of retinitis pigmentosa type 17 (RP17). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP17 inheritance is autosomal dominant. Defective acid overload removal from retina and retinal epithelium, due to mutant CA4, is responsible for photoreceptor degeneration, indicating that impaired pH homeostasis is the most likely cause underlying the RP17 phenotype. Belongs to the alpha-carbonic anhydrase family.

Protein type: Energy Metabolism - nitrogen; Lyase; Membrane protein, GPI anchor; EC 4.2.1.1

Chromosomal Location of Human Ortholog: 17q23

Cellular Component: Golgi apparatus; cell surface; rough endoplasmic reticulum; sarcoplasmic reticulum; brush border membrane; basolateral plasma membrane; secretory granule membrane; ER-Golgi intermediate compartment; trans-Golgi network; anchored to external side of plasma membrane; membrane; perinuclear region of cytoplasm; apical plasma membrane; plasma membrane; sarcolemma

Molecular Function: protein binding; carbonate dehydratase activity; zinc ion binding

Biological Process: response to drug; response to steroid hormone stimulus; bicarbonate transport; organ development; one-carbon compound metabolic process

Disease: Retinitis Pigmentosa 17