SDS-PAGE
Carbonic Anhydrase IX / Car9 Recombinant Protein | Car9 recombinant protein
Recombinant Mouse Carbonic Anhydrase IX / Car9 Protein (His tag)
Gene Names
Car9; Ca9; CAIX; MN/CA9
Purity
> 95 % as determined by SDS-PAGE
Synonyms
Carbonic Anhydrase IX / Car9; Recombinant Mouse Carbonic Anhydrase IX / Car9 Protein (His tag); RP23-195K8.9; CAIX; Ca9; MN/CA9; Car9 recombinant protein
Host
Human Cells
Purity/Purification
> 95 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH 7.4
Sequence Length
437
Application Notes
The secreted recombinant mouse CAR9 comprises 370 amino acids and has a calculated molecular mass of 40.3 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 45-50 kDa in SDS-PAGE under reducing conditions.
Predicted N Terminal
Gln 32
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
SDS-PAGE
SDS-PAGE
Related Product Information for Car9 recombinant protein
Background: Carbonic anhydrases IX (CA IX), also known as membrane antigen MN or CA9, is a member of the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide (H2O + CO2 = H+ + HCO3-) and thus participate in a variety of biological and physical processes. CA IX protein is expressed primarily in carcinoma cells lines, and the expression is cell density dependent and has been shown to be strongly induced by hypoxia, accordingly facilitates adaptation of tumor cells to hypoxic conditions. It is involved in tumorigenesis through many pathways, such as pH regulation and cell adhesion control. CA IX is used as a marker of tumor hypoxia and as a new therapeutic target for many human carcinomas and cancers.
Description: A DNA sequence encoding the extracellular domain of mouse CAR9 (NP_647466.2) (Met 1-Asp 390) was expressed, with a C-terminal polyhistidine tag.
Description: A DNA sequence encoding the extracellular domain of mouse CAR9 (NP_647466.2) (Met 1-Asp 390) was expressed, with a C-terminal polyhistidine tag.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,247 Da
NCBI Official Full Name
carbonic anhydrase 9
NCBI Official Synonym Full Names
carbonic anhydrase 9
NCBI Official Symbol
Car9
NCBI Official Synonym Symbols
Ca9; CAIX; MN/CA9
NCBI Protein Information
carbonic anhydrase 9
UniProt Protein Name
Carbonic anhydrase 9
Protein Family
UniProt Gene Name
Ca9
UniProt Synonym Gene Names
Car9; CA-IX; CAIX
Uniprot Description
CA9: Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. Forms oligomers linked by disulfide bonds. By hypoxia. Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa. Inhibited by coumarins, saccharin, sulfonamide derivatives such as acetazolamide (AZA) and Foscarnet (phosphonoformate trisodium salt). Belongs to the alpha-carbonic anhydrase family.
Protein type: EC 4.2.1.1; Energy Metabolism - nitrogen; Lyase; Membrane protein, integral; Nucleolus
Chromosomal Location of Human Ortholog: 4|4 A5
Cellular Component: basolateral plasma membrane; plasma membrane
Biological Process: morphogenesis of an epithelium; secretion
Research Articles on Car9
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Product Notes
The Car9 ca9 (Catalog #AAA2546859) is a Recombinant Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The secreted recombinant mouse CAR9 comprises 370 amino acids and has a calculated molecular mass of 40.3 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 45-50 kDa in SDS-PAGE under reducing conditions. Researchers should empirically determine the suitability of the Car9 ca9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Carbonic Anhydrase IX / Car9, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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