Sheep Calmodulin ELISA Kit | CAM elisa kit

Sheep Calmodulin ELISA Kit

Cat. Num. AAA738261

• Gene Names: DyakCam; Cam; DyakGE12425; dyak_GLEANR_12680; GE12425
• Reactivity: Sheep
• Synonyms: Calmodulin; Sheep Calmodulin ELISA Kit; CAM elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This CAM ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep CAM. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CAM elisa kit

Principle of the Assay: CAM ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CAM antibody and an CAM-HRP conjugate. The assay sample and buffer are incubated together with CAM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CAM concentration since CAM from samples and CAM-HRP conjugate compete for the anti-CAM antibody binding site. Since the number of sites is limited, as more sites are occupied by CAM from the sample, fewer sites are left to bind CAM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CAM concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CAM elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 194177199
• NCBI GeneID: 6530156
• Molecular Weight: 16,811 Da
• NCBI Official Full Name: Cam
• NCBI Official Symbol: DyakCam
• NCBI Official Synonym Symbols: Cam; DyakGE12425; dyak_GLEANR_12680; GE12425
• NCBI Protein Information: GE12425 gene product from transcript GE12425-RA; Cam; Cam-PA; DyakCam-PA; DyakGE12425-PA
• UniProt Protein Name: Cam
• Protein Family: Calmodulin
• UniProt Gene Name: Cam
• UniProt Synonym Gene Names: DyakCam; Dyak_GE12425; GE12425
• UniProt Entry Name: B4P5L3_DROYA