Rabbit HAAO Polyclonal Antibody | anti-HAAO antibody

HAAO Antibody

Cat. Num. AAA9406597

• Gene Names: HAAO; HAO; 3-HAO
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: HAAO; Polyclonal Antibody; HAAO Antibody; 3-hydroxyanthranilate 3; 4-dioxygenase; 3-hydroxyanthranilate oxygenase; 3-HAO; HAD; HAO; anti-HAAO antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 286

• Applicable Applications for anti-HAAO antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 17 amino acid peptide near the amino terminus of human HAAO.
• Target Name: HAAO
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of HAAO in Mouse liver tissue lysate with HAAO antibody at 1 ug/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of HAAO in human liver tissue with HAAO antibody at 2.5 ug/mL.)

Related Product Information for anti-HAAO antibody

HAAO (3-Hydroxyanthranilate 3, 4-dioxygenase) is a monomeric cytosolic protein of the family of intramolecular dioxygenases containing non-heme ferrous iron. It is widely distributed in peripheral organs, such as liver and kidney, and is present in low amounts in the central nervous system. This enzyme participates in tryptophan metabolism. It employs one cofactor, iron. HAAO catalyzes the synthesis of quinolinic acid (QUIN) from 3-hydroxyanthranilic acid. QUIN is an excitotoxin whose toxicity is mediated by its ability to activate glutamate N-methyl-D-aspartate receptors. Increased cerebral levels of QUIN may participate in the pathogenesis of neurological and inflammatory disorders. HAAO has been suggested to play a role in disorders associated with altered tissue levels of QUIN. Furthermore, recent study shows that HAAO are excellent candidate biomarkers for detecting ovarian cancer.

Product Categories/Family for anti-HAAO antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 188497625
• NCBI GeneID: 23498
• NCBI Accession #: NP_036337
• NCBI GenBank Nucleotide #: NM_012205.2
• Molecular Weight: 17,275 Da
• NCBI Official Full Name: 3-hydroxyanthranilate 3,4-dioxygenase
• NCBI Official Synonym Full Names: 3-hydroxyanthranilate 3,4-dioxygenase
• NCBI Official Symbol: HAAO
• NCBI Official Synonym Symbols: HAO; 3-HAO
• NCBI Protein Information: 3-hydroxyanthranilate 3,4-dioxygenase
• UniProt Protein Name: 3-hydroxyanthranilate 3,4-dioxygenase
• UniProt Gene Name: HAAO
• UniProt Synonym Gene Names: 3-HAO; HAD
• UniProt Entry Name: 3HAO_HUMAN

NCBI Description

3-Hydroxyanthranilate 3,4-dioxygenase is a monomeric cytosolic protein belonging to the family of intramolecular dioxygenases containing nonheme ferrous iron. It is widely distributed in peripheral organs, such as liver and kidney, and is also present in low amounts in the central nervous system. HAAO catalyzes the synthesis of quinolinic acid (QUIN) from 3-hydroxyanthranilic acid. QUIN is an excitotoxin whose toxicity is mediated by its ability to activate glutamate N-methyl-D-aspartate receptors. Increased cerebral levels of QUIN may participate in the pathogenesis of neurologic and inflammatory disorders. HAAO has been suggested to play a role in disorders associated with altered tissue levels of QUIN. [provided by RefSeq, Jul 2008]

Uniprot Description

HAAO: Catalyzes the oxidative ring opening of 3- hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate. Belongs to the 3-HAO family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; Amino Acid Metabolism - tryptophan; EC 1.13.11.6

Chromosomal Location of Human Ortholog: 2p21

Cellular Component: mitochondrial membrane; cytosol

Molecular Function: electron carrier activity; ferrous iron binding; oxygen binding; 3-hydroxyanthranilate 3,4-dioxygenase activity

Biological Process: anthranilate metabolic process; response to cadmium ion; response to zinc ion; quinolinate biosynthetic process; tryptophan catabolic process