Mouse anti-Human CD155/PVR Monoclonal Antibody | anti-CD155/PVR antibody

CD155/PVR (Extracellular region) Antibody

Cat. Num. AAA474238

• Gene Names: PVR; PVS; HVED; CD155; NECL5; TAGE4; Necl-5
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunoprecipitation, Immunocytochemistry
• Purity: Protein G chromatography
• Synonyms: CD155/PVR; Monoclonal Antibody; CD155/PVR (Extracellular region) Antibody; CD155; anti-CD155/PVR antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: M048
• Specificity: Clone M048 mouse monoclonal antibody detects a 75kDa* protein on SDS-PAGE "Native" or denatured immunoblots of human A549, NCI-H466, NCI-H1299, MDA-MB-231 and A431 carcinomas. The antibody detects CD155 in the membranes and cytoplasm in NCI-H446 cells after immunocytochemical labeling. The antibody works for western blot, immunoprecipitation, immunocytochemistry, and ELISA capture.
• Purity/Purification: Protein G chromatography
• Form/Format: Mouse monoclonal antibody purified with protein G chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-CD155/PVR antibody: Western Blot (WB), ELISA (EIA)Cap, Immunoprecipitation (IP), Immunocytochemistry (ICC)
• Application Notes: ELISA (Cap): 1:100; ICC: 1:100; IP: 1:100.; WB: 1:1000
• Immunogen: Clone (M048) was generated from a recombinant protein that included the extracellular region of human CD155 protein.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot of human A549 lung carcinoma (lane 1), NCI-H446 small cell lung carcinoma (lane 2), NCI-H1299 lung carcinoma (lane 3), MDA-MB-231 breast carcinoma (lane 4), and A431 epidermoid carcinoma (lane 5). The blot was probed with mouse monoclonal anti-CD155 (CM0481) at 1:500.)

Immunocytochemistry (ICC)

(Immunocytochemical labeling of CD155 in aldehyde fixed and NP-40 permeabilized human NCI-H446 small cell lung carcinoma cells. The cells were labeled with mouse monoclonal anti-CD155 (CM0481). The antibody was detected using goat anti-mouse DyLight 594.)

Related Product Information for anti-CD155/PVR antibody

CD155/Poliovirus receptor (PVR)/nectin-like 5 (Necl-5) is a transmembrane glycoprotein with extracellular immunoglobulin like domains, and an intracellular immunoreceptor tyrosine-based inhibitor motif (ITIM). CD155 was originally described as a mediator of poliovirus attachment to cells, but has also been implicated in adherens junction formation. CD155 binds nectin-3, and interacts with integrin alphavbeta3 and PDGFR to regulate integrin clustering and focal contact formation at the leading edge of migrating cells. CD155 is also a ligand for immunoreceptors that regulate tumor surveillance. CD155 binds DNAX-associated molecule 1 (DNAX-1), an activating receptor on natural killer cells and cytotoxic T-cells. Alternatively, CD155 may bind TIGIT immunoreceptor inducing an immunosuppressive and non-cytotoxic profile. In cancers, CD155 expression has been associated with unfavorable prognosis in colon cancer, breast cancer, lung adenocarcinoma, pancreatic cancer, melanoma, and glioblastoma. Cancer therapies have targeted CD155 interactions with TIGIT, and have used CD155 as a point of entry for recombinant oncolytic polioviruses.

References

Gao, J. (2017) Cancer Sci. 108(10):1934.
Molfetta, R. (2020) Int J Mol Sci. 21(3):922.

NCBI and Uniprot Product Information

• NCBI GI #: 609878250
• NCBI GeneID: 5817
• NCBI Accession #: NP_006496.4
• NCBI GenBank Nucleotide #: NM_006505.4
• UniProt Accession #: P15151; P15152; Q15267; Q15268; Q96BJ1; B4DTS9
• Molecular Weight: 45,303 Da
• NCBI Official Full Name: poliovirus receptor isoform alpha
• NCBI Official Synonym Full Names: poliovirus receptor
• NCBI Official Symbol: PVR
• NCBI Official Synonym Symbols: PVS; HVED; CD155; NECL5; TAGE4; Necl-5
• NCBI Protein Information: poliovirus receptor; nectin-like protein 5
• UniProt Protein Name: Poliovirus receptor
• UniProt Gene Name: PVR
• UniProt Synonym Gene Names: PVS; NECL-5
• UniProt Entry Name: PVR_HUMAN

NCBI Description

The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

PVR: Mediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors: CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration. Serves as a receptor for poliovirus attachment to target cells. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport. Belongs to the nectin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Immunoglobulin superfamily; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: extracellular space; cell surface; focal adhesion; cytoplasm; plasma membrane; integral to membrane

Molecular Function: viral receptor activity; protein binding; receptor activity; cell adhesion molecule binding

Biological Process: intercellular junction assembly and maintenance; regulation of immune response; cell migration; entry of virus into host cell; cell-cell adhesion; positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; response to virus; susceptibility to natural killer cell mediated cytotoxicity; positive regulation of natural killer cell mediated cytotoxicity