CALML5 recombinant protein

CALML5 Protein, Human, Recombinant (His & GST Tag)

Cat. Num. AAA8120970

• Gene Names: CALML5; CLSP
• Purity: >92% as determined by SDS-PAGE
• Synonyms: CALML5; CALML5 Protein; Human; Recombinant (His & GST Tag); Human CALML5/CLSP Protein (His & GST Tag); CLSP Protein; calmodulin like 5; CALML5 recombinant protein

• Host: E Coli
• Purity/Purification: >92% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 150mM NaCl, 1mM DTT, 0.5mM GSH, 10% glycerol, pH7.8. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy
• Sequence: Met1-Glu146

• Species: Human
• Predicted N Terminal: Met
• Tag: N-GST & His
• Protein Construction: A DNA sequence encoding the human CALML5 (AAH39172.1)(Met 1-Glu 146) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CALML5 recombinant protein

Background: Calmodulin-like protein 5, also known as Calmodulin-like skin protein, CALML5 and CLSP, is a protein which contains fourEF-hand domains. CALML5/CLSP is particularly abundant in the epidermis where its expression is directly related to keratinocyte differentiation.The expression is very low in lung. CALML5/CLSP binds calcium. It may be involved in terminal differentiation of keratinocytes. Coxsackievirus and adenovirus receptor (CAR) is a member of the immunoglobulin (Ig) superfamily and a component of epithelial tight junction. CAR functions as a primary receptor for coxsackievirus B and adenovirus (Ad) infection. CALML5/CLSP is closely related to CAR. The structure and dynamics of human calmodulin-like skin protein CALML5/CLSP have been characterized by NMR spectroscopy. The mobility of CALML5/CLSP has been found to be different for the N-terminal and C-terminal domains. The N-terminal domain is characterized by four stable helices, which experience large fluctuations. This is shown to be due to mutations in the hydrophobic core. The overall N-terminal domain behavior is similar both in the full-length protein and in the isolated domain.

Product Categories/Family for CALML5 recombinant protein

Actin Dynamics Signaling Pathway

References

Mehul B., et al., 2000, J. Biol. Chem. 275:12841-12847. Babini E., et al., 2006, Structure 14:1029-1038. Kawabata,K. et al., 2007, Gene Ther. 14 (16):1199-207.

NCBI and Uniprot Product Information

• NCBI GI #: 24657605
• NCBI GeneID: 51806
• Molecular Weight: 15,893 Da
• NCBI Official Full Name: Calmodulin-like 5
• NCBI Official Synonym Full Names: calmodulin like 5
• NCBI Official Symbol: CALML5
• NCBI Official Synonym Symbols: CLSP
• NCBI Protein Information: calmodulin-like protein 5

NCBI Description

This gene encodes a novel calcium binding protein expressed in the epidermis and related to the calmodulin family of calcium binding proteins. Functional studies with recombinant protein demonstrate it does bind calcium and undergoes a conformational change when it does so. Abundant expression is detected only in reconstructed epidermis and is restricted to differentiating keratinocytes. In addition, it can associate with transglutaminase 3, shown to be a key enzyme in the terminal differentiation of keratinocytes. [provided by RefSeq, Jul 2008]