Immunohistochemistry (IHC)
(Immunochemical staining of human CALML5 in human skin (from donor 2) with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to epithelium.)
Rabbit anti-Human CALML5 Polyclonal Antibody | anti-CALML5 antibody
Anti-CALML5 Antibody, Rabbit Polyclonal
Gene Names
CALML5; CLSP
Reactivity
Human
Applications
ELISA, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
CALML5; Polyclonal Antibody; Anti-CALML5 Antibody; Rabbit Polyclonal; CALML5 Antibody; Rabbit PAb; Antigen Affinity Purified; calmodulin like 5; Anti-CLSP Antibody; anti-CALML5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human CALML5
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CALML5 antibody
ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
ELISA: 1:5000-1:10,000
IHC-P: 1:500-1:2000
IHC-P: 1:500-1:2000
Immunogen
Recombinant Human CALML5 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human CALML5 (rh CALML5). CALML5 specific IgG was purified by Human CALML5 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of human CALML5 in human skin (from donor 2) with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to epithelium.)
Immunohistochemistry (IHC)
(Immunochemical staining of human CALML5 in human skin (from donor 2) with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to epithelium.)
Immunohistochemistry (IHC)
(Immunochemical staining of human CALML5 in human skin (from donor 1) with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to epithelium. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)
Immunohistochemistry (IHC)
(Immunochemical staining of human CALML5 in human skin (from donor 1) with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to epithelium. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)
Immunohistochemistry (IHC)
(Immunochemical staining of human CALML5 in human mammary adenocarcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to epithelium.)
Immunohistochemistry (IHC)
(Immunochemical staining of human CALML5 in human mammary adenocarcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to epithelium.)
Related Product Information for anti-CALML5 antibody
Calmodulin-like protein 5, also known as Calmodulin-like skin protein, CALML5 and CLSP, is a protein which contains fourEF-hand domains. CALML5/CLSP is particularly abundant in the epidermis where its expression is directly related to keratinocyte differentiation.The expression is very low in lung. CALML5/CLSP binds calcium. It may be involved in terminal differentiation of keratinocytes. Coxsackievirus and adenovirus receptor (CAR) is a member of the immunoglobulin (Ig) superfamily and a component of epithelial tight junction. CAR functions as a primary receptor for coxsackievirus B and adenovirus (Ad) infection. CALML5/CLSP is closely related to CAR. The structure and dynamics of human calmodulin-like skin protein CALML5/CLSP have been characterized by NMR spectroscopy. The mobility of CALML5/CLSP has been found to be different for the N-terminal and C-terminal domains. The N-terminal domain is characterized by four stable helices, which experience large fluctuations. This is shown to be due to mutations in the hydrophobic core. The overall N-terminal domain behavior is similar both in the full-length protein and in the isolated domain.
Product Categories/Family for anti-CALML5 antibody
References
Mehul B., et al., 2000, J. Biol. Chem. 275:12841-12847. Babini E., et al., 2006, Structure 14:1029-1038. Kawabata, K. et al., 2007, Gene Ther. 14 (16):1199-207.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a novel calcium binding protein expressed in the epidermis and related to the calmodulin family of calcium binding proteins. Functional studies with recombinant protein demonstrate it does bind calcium and undergoes a conformational change when it does so. Abundant expression is detected only in reconstructed epidermis and is restricted to differentiating keratinocytes. In addition, it can associate with transglutaminase 3, shown to be a key enzyme in the terminal differentiation of keratinocytes. [provided by RefSeq, Jul 2008]
Research Articles on CALML5
Similar Products
Product Notes
The CALML5 (Catalog #AAA8105804) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CALML5 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CALML5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P). ELISA: 1:5000-1:10,000 IHC-P: 1:500-1:2000. Researchers should empirically determine the suitability of the CALML5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CALML5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
