CALML3 recombinant protein

CALML3 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121178

• Gene Names: CALML3; CLP
• Purity: >95% as determined by SDS-PAGE
• Synonyms: CALML3; CALML3 Protein; Human; Recombinant (His Tag); Human CALML3 Protein (His Tag); CLP Protein; calmodulin-like 3; CALML3 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Lys149

• Species: Human
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human CALML3 (P27482)(Met 1-Lys 149) was expressed, with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CALML3 recombinant protein

Background: Calmodulin-like protein 3 (CALML3) is similar to that of authentic calmodulin and may actually compete with calmodulin by binding, with different affinity, to cellular substrates. Calmodulin-like protein 3 (CALML3) is a tumor-sensitive protein specifically expressed in normal epithelial cells but downregulated in tumorigenesis. Downregulation of the protein is an early event in breast cancer development. One of the most pressing questions raised by the discovery of CLP/CALML3 is that of its potential targets. Although it is 85% identical to human calmodulin, the distinct properties of CLP suggest that it has specific targets or targets that only partially overlap with those of calmodulin. Research has identified the unconventional myosin-10 (Myo10) as a specific target of CALML3. The discovery of Myo10 as a specific target of CALML3 is highly significant and suggests multiple lines of further research such as investigations of the Ca2+ regulation of Myo10 and the role of the loss of CLP in epithelial differentiation, adhesion, and cancer. Cells expressing CALML3 displayed a striking increase in the number and length of myosin-10-positive filopodia and showed increased mobility in a wound healing assay.

Product Categories/Family for CALML3 recombinant protein

Actin Dynamics Signaling Pathway

References

Bennett RD, et al. (2007) Calmodulin-like protein increases filopodia-dependent cell motility via up-regulation of myosin-10. J Biol Chem. 282(5): 3205-12.Perochon A, et al. (2011) Calmodulin and calmodulin-like proteins in plant calcium signaling. Biochimie. 93(12): 2048-53.Chinpongpanich A, et al. (2011) Biophysical characterization of calmodulin and calmodulin-like proteins from rice, Oryza sativa L. Acta Biochim Biophys Sin (Shanghai). 43(11): 867-76.

NCBI and Uniprot Product Information

• NCBI GI #: 4885111
• NCBI GeneID: 810
• NCBI Accession #: NP_005176.1
• NCBI GenBank Nucleotide #: NM_005185.3
• UniProt Accession #: P27482; Q5SQI4; B2R9V6
• Molecular Weight: 16,891 Da
• NCBI Official Full Name: calmodulin-like protein 3
• NCBI Official Synonym Full Names: calmodulin-like 3
• NCBI Official Symbol: CALML3
• NCBI Official Synonym Symbols: CLP
• NCBI Protein Information: calmodulin-like protein 3; caM-like protein; calmodulin-related protein NB-1
• UniProt Protein Name: Calmodulin-like protein 3
• Protein Family: Calmodulin-like protein
• UniProt Gene Name: CALML3
• UniProt Synonym Gene Names: CLP
• UniProt Entry Name: CALL3_HUMAN

Uniprot Description

CALML3: May be similar to that of authentic calmodulin and may actually compete with calmodulin by binding, with different affinities, to cellular substrates. Belongs to the calmodulin family.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 10p15.1

Molecular Function: protein binding; calcium ion binding