EphB6 active protein

EphB6 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8123143

• Gene Names: Ephb6; Mep; Cekl
• Purity: >85% as determined by SDS-PAGE
• Synonyms: EphB6; EphB6 Protein; Mouse; Recombinant (His Tag); Mouse EphB6 Protein (His Tag); Cekl Protein; Mep Protein; EPH receptor B6; EphB6 active protein

• Host: HEK293 Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser587

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Leu 33
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse EPHB6 (O08644-1)(Met1-Ser587) was expressed with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: 1. Immobilized mouse EPHB6-His at 10ug/ml (100uL/well) can bind mouse EFNB1-Fc. The EC50 of mouse EFNB1-Fc is 0.12-0.28ug/ml.; 2. Immobilized mouse EPHB6-His at 10ug/ml (100uL/well) can bind mouse EFNB2-Fc. The EC50 of mouse EFNB2-Fc is 0.04-0.08ug/ml.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for EphB6 active protein

Background: Ephrins are divided into the ephrin-A (EFNA) class and the ephrin-B (EFNB) class based on their structures and sequence relationships. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB6 is an unusual Eph receptor, lacking catalytic capacity due to alterations in its kinase domain. Interestingly, increased metastatic activity is associated with reduced EphB6 receptor expression in several tumor types, including breast cancer. This emphasizes the potential of EphB6 to act as a suppressor of cancer aggressiveness. EphB6 suppress cancer invasiveness through c-Cbl-dependent signaling, morphologic changes, and cell attachment and indicate that EphB6 may represent a useful prognostic marker and a promising target for therapeutic approaches. EphB6 can both positively and negatively regulate cell adhesion and migration, and suggest that tyrosine phosphorylation of the receptor by an Src family kinase acts as the molecular switch for the functional transition. In addition, Ephrin-B2 may be a physiological ligand for the EphB6 receptor.

Product Categories/Family for EphB6 active protein

Actin Dynamics Signaling Pathway

References

Munthe E, et al. (2000)Ephrin-B2 is a candidate ligand for the Eph receptor, EphB6. FEBS Lett. 466(1): 169-74.Matsuoka H, et al. (2005) Biphasic functions of the kinase-defective Ephb6 receptor in cell adhesion and migration. J Biol Chem. 280(32): 29355-63.Truitt L, et al. (2010) The EphB6 receptor cooperates with c-Cbl to regulate the behavior of breast cancer cells. Cancer Res. 70(3): 1141-53.

NCBI and Uniprot Product Information

• NCBI GI #: 226437671
• NCBI GeneID: 13848
• NCBI Accession #: NP_001139823.1
• NCBI GenBank Nucleotide #: NM_001146351.1
• UniProt Accession #: O08644; Q3TQ77; Q8BN76; Q8K0A9
• Molecular Weight: 110,107 Da
• NCBI Official Full Name: ephrin type-B receptor 6
• NCBI Official Synonym Full Names: Eph receptor B6
• NCBI Official Symbol: Ephb6
• NCBI Official Synonym Symbols: Mep; Cekl
• NCBI Protein Information: ephrin type-B receptor 6; ephrin type A receptor; chicken eph/elk receptor-like protein; tyrosine-protein kinase-defective receptor EPH-6
• UniProt Protein Name: Ephrin type-B receptor 6
• Protein Family: Ephrin type-B receptor
• UniProt Gene Name: Ephb6
• UniProt Synonym Gene Names: Cekl
• UniProt Entry Name: EPHB6_MOUSE

Uniprot Description

Function: Kinase-defective receptor for members of the ephrin-B family. Binds to ephrin-B1 and ephrin-B2. Modulates cell adhesion and migration by exerting both positive and negative effects upon stimulation with ephrin-B2. Inhibits JNK activation, T-cell receptor-induced IL-2 secretion and CD25 expression upon stimulation with ephrin-B2

By similarity.

Subunit structure: Interacts with CBL and EPHB1. Interacts with FYN; this interaction takes place in a ligand-independent manner

By similarity.

Subcellular location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. Isoform 3: Secreted.

Tissue specificity: High level in thymus, and brain. Very low levels of expression in kidney, lung, liver, bone marrow, skeletal muscle, spleen from 2 week old and adult mice, heart, testes and embryonic stem cells. Ref.1

Domain: The protein kinase domain is predicted to be catalytically inactive. Its extracellular domain is capable of promoting cell adhesion and migration in response to low concentrations of ephrin-B2, but its cytoplasmic domain is essential for cell repulsion and inhibition of migration induced by high concentrations of ephrin-B2

By similarity.

Post-translational modification: Ligand-binding increases phosphorylation on tyrosine residues. Phosphorylation on tyrosine residues is mediated by transphosphorylation by the catalytically active EPHB1 in a ligand-independent manner. Tyrosine phosphorylation of the receptor may act as a switch on the functional transition from cell adhesion/attraction to de-adhesion/repulsion

By similarity.

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.Contains 1 Eph LBD (Eph ligand-binding) domain.Contains 2 fibronectin type-III domains.Contains 1 protein kinase domain.Contains 1 SAM (sterile alpha motif) domain.