SDS-PAGE
Carbonic Anhydrase XIV / Car14 Recombinant Protein | Car14 recombinant protein
Recombinant Mouse Carbonic Anhydrase XIV / Car14 Protein (His tag)
Gene Names
Car14; Ca14; AW536446
Purity
> 95 % as determined by SDS-PAGE
Synonyms
Carbonic Anhydrase XIV / Car14; Recombinant Mouse Carbonic Anhydrase XIV / Car14 Protein (His tag); Car14; Catm; Ca14; Car14 recombinant protein
Host
Human Cells
Purity/Purification
> 95 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 25mM Tris, 0.15mM NaCl, pH 7.5
Sequence Length
337
Application Notes
The recombinant mouse CA14 consists of 286 amino acids and has a calculated molecular mass of 32.2 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 45-48 kDa protein in SDS-PAGE under reducing conditions.
Predicted N Terminal
Ala 16
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
SDS-PAGE
SDS-PAGE
Related Product Information for Car14 recombinant protein
Background: The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. CAXIV is a member of CA family that showed an overall similarity of 29-46% to other active CA isozymes. The highest percentage similarity was with a transmembrane CA isoform, CAXII. The CAXIV was found high concentrations in human heart, brain, liver, and skeletal muscle but lower in the colon, small intestine, urinary bladder, and kidney. No CAXIV mRNA was seen in the salivary gland and pancreas. CAXIV is a likely candidate for the extracellular CA postulated to have an important role in modulating excitatory synaptic transmission in brain.
Description: A DNA sequence encoding the extracellular domain of mouse CA14 (NP_035927.1) (Met 1-Met 290) was expressed with a C-terminal polyhistidine tag.
Description: A DNA sequence encoding the extracellular domain of mouse CA14 (NP_035927.1) (Met 1-Met 290) was expressed with a C-terminal polyhistidine tag.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37,505 Da
NCBI Official Full Name
carbonic anhydrase 14
NCBI Official Synonym Full Names
carbonic anhydrase 14
NCBI Official Symbol
Car14
NCBI Official Synonym Symbols
Ca14; AW536446
NCBI Protein Information
carbonic anhydrase 14
UniProt Protein Name
Carbonic anhydrase 14
UniProt Gene Name
Ca14
UniProt Synonym Gene Names
Car14; Catm; CA-XIV
Uniprot Description
CA14: Reversible hydration of carbon dioxide. Belongs to the alpha-carbonic anhydrase family.
Protein type: EC 4.2.1.1; Energy Metabolism - nitrogen; Lyase; Membrane protein, integral
Chromosomal Location of Human Ortholog: 3|3 F2.1
Molecular Function: carbonate dehydratase activity
Biological Process: carbon dioxide transport; regulation of pH
Research Articles on Car14
Similar Products
Product Notes
The Car14 ca14 (Catalog #AAA2547214) is a Recombinant Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The recombinant mouse CA14 consists of 286 amino acids and has a calculated molecular mass of 32.2 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 45-48 kDa protein in SDS-PAGE under reducing conditions. Researchers should empirically determine the suitability of the Car14 ca14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Carbonic Anhydrase XIV / Car14, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
