Immunofluorescence (IF)
(Immunofluorescence staining of Human CA14 in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human CA14 monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane.)
Rabbit anti-Human Carbonic Anhydrase XIV Monoclonal Antibody | anti-CA14 antibody
Recombinant Anti-Carbonic Anhydrase XIV Antibody, Rabbit Monoclonal
No cross-reactivity in ELISA with Human CARPX, Human CA12, Mouse CA14
IHC-P: 1:100-1:500
ICC/IF: 1:20-1:100
Immunofluorescence (IF)
(Immunofluorescence staining of Human CA14 in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human CA14 monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane.)
Immunofluorescence (IF)
(Immunofluorescence staining of Human CA14 in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human CA14 monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane.)
Immunohistochemistry (IHC)
(Immunochemical staining of human CA14 in human liver with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of hepatocyte.)
Immunohistochemistry (IHC)
(Immunochemical staining of human CA14 in human liver with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of hepatocyte.)
NCBI and Uniprot Product Information
NCBI Description
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA XIV is predicted to be a type I membrane protein and shares highest sequence similarity with the other transmembrane CA isoform, CA XII; however, they have different patterns of tissue-specific expression and thus may play different physiologic roles. [provided by RefSeq, Jul 2008]
Uniprot Description
CA14: Reversible hydration of carbon dioxide. Belongs to the alpha-carbonic anhydrase family.
Protein type: Lyase; EC 4.2.1.1; Membrane protein, integral; Energy Metabolism - nitrogen
Chromosomal Location of Human Ortholog: 1q21
Cellular Component: integral to membrane; plasma membrane
Molecular Function: carbonate dehydratase activity; metal ion binding
Biological Process: bicarbonate transport
Research Articles on CA14
Similar Products
Product Notes
The CA14 ca14 (Catalog #AAA8102873) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-Carbonic Anhydrase XIV Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Carbonic Anhydrase XIV can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF). ELISA: 1:5000-1:10,000 IHC-P: 1:100-1:500 ICC/IF: 1:20-1:100. Researchers should empirically determine the suitability of the CA14 ca14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Carbonic Anhydrase XIV, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
