Carbonic Anhydrase XIV Active Protein | CA14 active protein

Carbonic Anhydrase XIV Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122459

• Gene Names: Car14; Ca14; CA-XIV; AW536446
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Carbonic Anhydrase XIV; Carbonic Anhydrase XIV Protein; Mouse; Recombinant (His Tag); Mouse Carbonic Anhydrase XIV/Car14 Protein (His Tag); Ca14 Protein; Car14 Protein; Catm Protein; carbonic anhydrase XIV; CA14 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 25mM Tris, 0.15mM NaCl, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Met290

• Species: Mouse
• Activity: Measured by its esterase activity. The specific activity is >400pmoles/min/ug, as measured with 1mM 4-Nitrophenyl acetate and 0.8 ug enzyme at 400nM in 100 uL of 12.5mM Tris, 75mM NaCl, pH7.5.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ala 16
• Tag: C-His
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse CA14 (NP_035927.1)(Met 1-Met 290) was expressed with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CA14 active protein

Background: The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. CAXIV is a member of CA family that showed an overall similarity of 29-46% to other active CA isozymes. The highest percentage similarity was with a transmembrane CA isoform, CAXII. The CAXIV was found high concentrations in human heart, brain, liver, and skeletal muscle but lower in the colon, small intestine, urinary bladder, and kidney. No CAXIV mRNA was seen in the salivary gland and pancreas. CAXIV is a likely candidate for the extracellular CA postulated to have an important role in modulating excitatory synaptic transmission in brain.

References

Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1): 1-20.Parkkila S, et al. (2001) Expression of membrane-associated carbonic anhydrase XIV on neurons and axons in mouse and human brain. PNAS. 98(4): 1918-23.

NCBI and Uniprot Product Information

• NCBI GI #: 6753264
• NCBI GeneID: 23831
• NCBI Accession #: NP_035927.1
• NCBI GenBank Nucleotide #: NM_011797.3
• UniProt Accession #: Q9WVT6
• Molecular Weight: 37,505 Da
• NCBI Official Full Name: carbonic anhydrase 14 isoform 1
• NCBI Official Synonym Full Names: carbonic anhydrase 14
• NCBI Official Symbol: Car14
• NCBI Official Synonym Symbols: Ca14; CA-XIV; AW536446
• NCBI Protein Information: carbonic anhydrase 14
• UniProt Protein Name: Carbonic anhydrase 14
• Protein Family: Carbonic anhydrase
• UniProt Gene Name: Ca14
• UniProt Synonym Gene Names: Car14; Catm; CA-XIV

Uniprot Description

CA14: Reversible hydration of carbon dioxide. Belongs to the alpha-carbonic anhydrase family.

Protein type: EC 4.2.1.1; Energy Metabolism - nitrogen; Lyase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3|3 F2.1

Cellular Component: integral component of membrane; membrane

Molecular Function: carbonate dehydratase activity; lyase activity; metal ion binding

Biological Process: carbon dioxide transport; regulation of pH