Rabbit anti-Human ABCC4 Polyclonal Antibody | anti-ABCC4 antibody

ABCC4, CT (Multi-specific Organic Anion Transporter B, MOAT-B, MRP/cMOAT-related ABC Transporter, ATP-binding Cassette Sub-family C Member 4, Multidrug Resistance-associated Protein 4, MRP4) (Biotin)

Cat. Num. AAA6487526

• Gene Names: ABCC4; MRP4; MOATB; MOAT-B
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: ABCC4; Polyclonal Antibody; CT (Multi-specific Organic Anion Transporter B; MOAT-B; MRP/cMOAT-related ABC Transporter; ATP-binding Cassette Sub-family C Member 4; Multidrug Resistance-associated Protein 4; MRP4) (Biotin); anti-ABCC4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ABCC4.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 1325

• Applicable Applications for anti-ABCC4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human ABCC4 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-ABCC4 Antibody (C-term) in CEM cell line lysates (35ug/lane). ABCC4 (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-ABCC4 antibody

Antibodies; Multi Drug Resistance (MDR)

References

1. Lee K., Belinsky M.G., Bell D.W.Cancer Res. 58:2741-2747(1998). 2. Adachi M., Sampath J., Lan L.B.J. Biol. Chem. 277:38998-39004(2002). 3. Kool M., de Haas M., Scheffer G.L.Cancer Res. 57:3537-3547(1997). 4. Janke D., Mehralivand S., Strand D.Hum. Mutat. 29:659-669(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 157502201
• NCBI GeneID: 10257
• NCBI Accession #: NP_005836
• NCBI GenBank Nucleotide #: NP_005836.2
• UniProt Accession #: O15439
• Molecular Weight: 149.527
• NCBI Official Full Name: multidrug resistance-associated protein 4 isoform 1
• NCBI Official Synonym Full Names: ATP binding cassette subfamily C member 4
• NCBI Official Symbol: ABCC4
• NCBI Official Synonym Symbols: MRP4; MOATB; MOAT-B
• NCBI Protein Information: multidrug resistance-associated protein 4
• UniProt Protein Name: Multidrug resistance-associated protein 4
• Protein Family: ABC transporter C family
• UniProt Gene Name: ABCC4
• UniProt Synonym Gene Names: MRP4; MOAT-B
• UniProt Entry Name: MRP4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This family member plays a role in cellular detoxification as a pump for its substrate, organic anions. It may also function in prostaglandin-mediated cAMP signaling in ciliogenesis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2014]

Uniprot Description

ABCC4: a widely-expressed multi-pass membrane protein. An organic anion pump involved in cellular detoxification. Widely expressed, with particularly high levels in prostate, but is barely detectable in liver.

Protein type: Transporter; Membrane protein, multi-pass; Transporter, ABC family; Membrane protein, integral

Chromosomal Location of Human Ortholog: 13q32

Cellular Component: membrane; basolateral plasma membrane; integral to membrane; plasma membrane; platelet dense granule membrane

Molecular Function: ATPase activity, coupled to transmembrane movement of substances; 15-hydroxyprostaglandin dehydrogenase (NAD+) activity; ATP binding

Biological Process: response to drug; platelet activation; platelet degranulation; positive regulation of smooth muscle cell proliferation; response to organic nitrogen; blood coagulation; transmembrane transport; response to organic cyclic substance