Carbonic Anhydrase XIV / CA14 Recombinant Protein | CA14 recombinant protein

Recombinant Human Carbonic Anhydrase XIV / CA14 Protein (His tag)

Cat. Num. AAA2546249

• Gene Names: CA14; CAXiV
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: Carbonic Anhydrase XIV / CA14; Recombinant Human Carbonic Anhydrase XIV / CA14 Protein (His tag); CAXiV; CA14; CA14 recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 337

• Application Notes: The recombinant human CA14 consisting of 286 amino acids and has a calculated molecular mass of 32.3 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 45-48 kDa protein in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Ala 16
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CA14 recombinant protein

Background: The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. CAXIV is a member of CA family that showed an overall similarity of 29-46% to other active CA isozymes. The highest percentage similarity was with a transmembrane CA isoform, CAXII. The CAXIV was found high concentrations in human heart, brain, liver, and skeletal muscle but lower in the colon, small intestine, urinary bladder, and kidney. No CAXIV mRNA was seen in the salivary gland and pancreas. CAXIV is a likely candidate for the extracellular CA postulated to have an important role in modulating excitatory synaptic transmission in brain.

Description: A DNA sequence encoding the extracellular domain of human CA14 (NP_036245.1) (Met 1-Met 290) was expressed with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 6912284
• NCBI GeneID: 23632
• NCBI Accession #: NP_036245.1
• NCBI GenBank Nucleotide #: NM_012113.2
• UniProt Accession #: Q9ULX7; Q5TB24; Q8NCF4
• Molecular Weight: 37,668 Da
• NCBI Official Full Name: carbonic anhydrase 14
• NCBI Official Synonym Full Names: carbonic anhydrase 14
• NCBI Official Symbol: CA14
• NCBI Official Synonym Symbols: CAXiV
• NCBI Protein Information: carbonic anhydrase 14
• UniProt Protein Name: Carbonic anhydrase 14
• Protein Family: Carbonic anhydrase
• UniProt Gene Name: CA14
• UniProt Synonym Gene Names: CA-XIV

NCBI Description

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA XIV is predicted to be a type I membrane protein and shares highest sequence similarity with the other transmembrane CA isoform, CA XII; however, they have different patterns of tissue-specific expression and thus may play different physiologic roles. [provided by RefSeq, Jul 2008]

Uniprot Description

CA14: Reversible hydration of carbon dioxide. Belongs to the alpha-carbonic anhydrase family.

Protein type: EC 4.2.1.1; Energy Metabolism - nitrogen; Lyase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q21.2

Cellular Component: plasma membrane

Molecular Function: carbonate dehydratase activity

Biological Process: bicarbonate transport