Western Blot (WB)
(Western Blot analysis of GBF1 antibody.)
Rabbit anti-Human, Mouse GBF1 Polyclonal Antibody | anti-GBF1 antibody
GBF1 Polyclonal Antibody
Gene Names
GBF1; ARF1GEF
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
GBF1; Polyclonal Antibody; GBF1 Polyclonal Antibody; ARF1GEF; anti-GBF1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
GBF1 polyclonal antibody detects endogenous levels of GBF1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
1856
Applicable Applications for anti-GBF1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Recombinant protein of human GBF1.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot analysis of GBF1 antibody.)
Western Blot (WB)
(Western Blot analysis of GBF1 antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human esophagus using GBF1 antibody at dilution of 1:100 (x40 lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human esophagus using GBF1 antibody at dilution of 1:100 (x40 lens).)
Related Product Information for anti-GBF1 antibody
This gene encodes a member of the Sec7 domain family. The encoded protein is a guanine nucleotide exchange factor that regulates the recruitment of proteins to membranes by mediating GDP to GTP exchange. The encoded protein is localized to the Golgi apparatus and plays a role in vesicular trafficking by activating ADP ribosylation factor 1. The encoded protein has also been identified as an important host factor for viral replication. Multiple transcript variants have been observed for this gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 260kDa
NCBI Official Full Name
Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 2
NCBI Official Synonym Full Names
golgi brefeldin A resistant guanine nucleotide exchange factor 1
NCBI Official Symbol
GBF1
NCBI Official Synonym Symbols
ARF1GEF
NCBI Protein Information
Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1
UniProt Protein Name
Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1
Protein Family
UniProt Gene Name
GBF1
UniProt Synonym Gene Names
KIAA0248; BFA-resistant GEF 1
UniProt Entry Name
GBF1_HUMAN
NCBI Description
This gene encodes a member of the Sec7 domain family. The encoded protein is a guanine nucleotide exchange factor that regulates the recruitment of proteins to membranes by mediating GDP to GTP exchange. The encoded protein is localized to the Golgi apparatus and plays a role in vesicular trafficking by activating ADP ribosylation factor 1. The encoded protein has also been identified as an important host factor for viral replication. Multiple transcript variants have been observed for this gene. [provided by RefSeq, Dec 2010]
Research Articles on GBF1
Similar Products
Product Notes
The GBF1 gbf1 (Catalog #AAA3009209) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GBF1 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GBF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the GBF1 gbf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GBF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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