VCP blocking peptide

VCP Antibody (C-term) Blocking Peptide

• Gene Names: VCP; p97; TERA; ALS14; CMT2Y; IBMPFD; HEL-220; IBMPFD1; HEL-S-70
• Synonyms: VCP; VCP Antibody (C-term) Blocking Peptide; Transitional endoplasmic reticulum ATPase; TER ATPase; 15S Mg(2+)-ATPase p97 subunit; Valosin-containing protein; VCP blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human VCP. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 806

• Cellular Location: Cytoplasm, cytosol. Endoplasmic reticulum. Nucleus. Note: Present in the neuronal hyaline inclusion bodies specifically found in motor neurons from amyotrophic lateral sclerosis patients. Present in the Lewy bodies specifically found in neurons from Parkinson disease patients. Recruited to the cytoplasmic surface of the endoplasmic reticulum via interaction with AMFR/gp78. Following DNA double-strand breaks, recruited to the sites of damage. Recruited to stalled replication forks via interaction with SPRTN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for VCP blocking peptide

Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. Regulates E3 ubiquitin-protein ligase activity of RNF19A. Component of the VCP/p97-AMFR/gp78 complex that participates in the final step of the sterol-mediated ubiquitination and endoplasmic reticulum-associated degradation (ERAD) of HMGCR. Also involved in DNA damage response: recruited to double-strand breaks (DSBs) sites in a RNF8- and RNF168-dependent manner and promotes the recruitment of TP53BP1 at DNA damage sites. Recruited to stalled replication forks by SPRTN: may act by mediating extraction of DNA polymerase eta (POLH) to prevent excessive translesion DNA synthesis and limit the incidence of mutations induced by DNA damage. Required for cytoplasmic retrotranslocation of stressed/damaged mitochondrial outer-membrane proteins and their subsequent proteasomal degradation.

NCBI and Uniprot Product Information

• NCBI GI #: 6094447
• NCBI GeneID: 7415
• UniProt Accession #: P55072; Q0V924; Q2TAI5; Q969G7; Q9UCD5; B2R5T8
• Molecular Weight: 89,322 Da
• NCBI Official Full Name: Transitional endoplasmic reticulum ATPase
• NCBI Official Synonym Full Names: valosin containing protein
• NCBI Official Symbol: VCP
• NCBI Official Synonym Symbols: p97; TERA; ALS14; CMT2Y; IBMPFD; HEL-220; IBMPFD1; HEL-S-70
• NCBI Protein Information: transitional endoplasmic reticulum ATPase
• UniProt Protein Name: Transitional endoplasmic reticulum ATPase
• Protein Family: VCP-like ATPase
• UniProt Gene Name: VCP
• UniProt Synonym Gene Names: TER ATPase; VCP
• UniProt Entry Name: TERA_HUMAN

NCBI Description

The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq, Jul 2008]

Uniprot Description

VCP: valosin-containing protein (VCP) is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the nuclear envelope and of the transitional endoplasmic reticulum (tER). Regulates NFKappaB pathway, which is important for metastasis of osteosarcoma. Tyrosine phosphorylation regulates its cell cycle-dependent nuclear localization.

Protein type: DNA repair, damage; EC 3.6.4.6; Chaperone; Hydrolase; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; endoplasmic reticulum membrane; intracellular membrane-bound organelle; lipid particle; nucleoplasm; nucleus; perinuclear region of cytoplasm; proteasome complex

Molecular Function: ATPase activity; polyubiquitin binding; protein binding; protein domain specific binding; protein phosphatase binding; ubiquitin protein ligase binding

Biological Process: bypass DNA synthesis; caspase activation; double-strand break repair; ER-associated protein catabolic process; establishment of protein localization; NADH metabolic process; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein catabolic process; positive regulation of protein complex assembly; protein amino acid N-linked glycosylation via asparagine; protein ubiquitination; regulation of apoptosis; response to DNA damage stimulus; retrograde protein transport, ER to cytosol; unfolded protein response; viral genome replication

Disease: Amyotrophic Lateral Sclerosis 14, With Or Without Frontotemporal Dementia; Inclusion Body Myopathy With Early-onset Paget Disease With Or Without Frontotemporal Dementia 1