AXIN1 recombinant protein

AXIN1 Protein

Cat. Num. AAA516574

• Gene Names: AXIN1; AXIN; PPP1R49
• Applications: Western Blot
• Synonyms: AXIN1; AXIN1 Protein; AXIN; MGC52315; AXIN1 recombinant protein

• Host: Sf9 insect cells
• Form/Format: Recombinant protein stored in 50mM Tris-HCl, pH7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

• Applicable Applications for AXIN1 recombinant protein: Western Blot (WB)
• Tag: GST
• Species: Human
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-Page

(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)

Related Product Information for AXIN1 recombinant protein

Full-length recombinant human AXIN1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. This gene accession number is BC044648.
AXIN 1 encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain that interacts with adenomatosis polyposis coli, catenin beta-1, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. AXIN1 has both positive and negative regulatory roles in Wnt-beta-catenin signaling. AXIN1 is a core component of a 'destruction complex' that promotes phosphorylation and polyubiquitination of cytoplasmic beta-catenin, resulting in beta-catenin proteasomal degradation in the absence of Wnt signaling. Nuclear accumulation of AXIN1 can positively influence beta-catenin-mediated transcription during Wnt signalling and can induce apoptosis (1). AXIN1 may be an effective therapeutic molecule for suppressing growth of hepatocellular and colorectal cancers (2).

Product Categories/Family for AXIN1 recombinant protein

Regulatory Proteins; Apoptosis/Autophagy; Cancer; Cell Cycle; JNK/SAPK Pathway; WNT Signaling

References

1. Lui, T. T. H.et.al: The ubiquitin-specific protease USP34 regulates axin stability and Wnt/beta-catenin signaling. Molec. Cell. Biol. 31: 2053-2065, 2011.
2. Satoh, S.et.al: AXIN1 mutations in hepatocellular carcinomas, and growth suppression in cancer cells by virus-mediated transfer of AXIN1. Nature Genet. 24: 245-250, 2000.

NCBI and Uniprot Product Information

• NCBI GI #: 27881697
• NCBI GeneID: 8312
• Molecular Weight: 91,653 Da
• NCBI Official Full Name: Homo sapiens axin 1, mRNA
• NCBI Official Synonym Full Names: axin 1
• NCBI Official Symbol: AXIN1
• NCBI Official Synonym Symbols: AXIN; PPP1R49
• NCBI Protein Information: axin-1
• Protein Family: Axin

NCBI Description

This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin beta-1, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]