Mouse TERA Monoclonal Antibody | anti-VCP antibody

TERA Antibody

Cat. Num. AAA9215476

• Gene Names: VCP; p97; TERA; ALS14; IBMPFD; HEL-220; IBMPFD1; HEL-S-70
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Synonyms: TERA; Monoclonal Antibody; TERA Antibody; Purified Mouse Monoclonal Antibody (Mab); Transitional endoplasmic reticulum ATPase; TER ATPase; 15S Mg(2+)-ATPase p97 subunit; Valosin-containing protein; VCP; anti-VCP antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: 1563CT163.48.77
• Specificity: This TERA antibody is generated from a mouse immunized with a recombinant protein of human TERA.
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Sequence Length: 806

• Applicable Applications for anti-VCP antibody: Western Blot (WB)
• Application Notes: WB ~~ 1:4000
• Antigen Source: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-TERA Antibody at 1:4000 dilutionLane 1: Hela whole cell lysateLane 2: K562 whole cell lysateLane 3: A549 whole cell lysateLane 4: C6 whole cell lysateLane 5: U-87 MG whole cell lysateLane 6: NIH/3T3 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 89 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-VCP antibody

Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. Regulates E3 ubiquitin-protein ligase activity of RNF19A. Component of the VCP/p97-AMFR/gp78 complex that participates in the final step of the sterol-mediated ubiquitination and endoplasmic reticulum-associated degradation (ERAD) of HMGCR. Also involved in DNA damage response: recruited to double-strand breaks (DSBs) sites in a RNF8- and RNF168-dependent manner and promotes the recruitment of TP53BP1 at DNA damage sites. Recruited to stalled replication forks by SPRTN: may act by mediating extraction of DNA polymerase eta (POLH) to prevent excessive translesion DNA synthesis and limit the incidence of mutations induced by DNA damage. Required for cytoplasmic retrotranslocation of stressed/damaged mitochondrial outer-membrane proteins and their subsequent proteasomal degradation.

Product Categories/Family for anti-VCP antibody

Crown Antibodies; Metabolism; Neuroscience; Signal Transduction

References

Lamerdin J.E.,et al.Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases. Hu R.-M.,et al.Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000). Ota T.,et al.Nat. Genet. 36:40-45(2004). Humphray S.J.,et al.Nature 429:369-374(2004). Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 6005942
• NCBI GeneID: 7415
• NCBI Accession #: NP_009057.1
• NCBI GenBank Nucleotide #: NM_007126.3
• UniProt Accession #: P55072; Q0V924; Q2TAI5; Q969G7; Q9UCD5; B2R5T8
• Molecular Weight: 89322
• NCBI Official Full Name: transitional endoplasmic reticulum ATPase
• NCBI Official Synonym Full Names: valosin containing protein
• NCBI Official Symbol: VCP
• NCBI Official Synonym Symbols: p97; TERA; ALS14; IBMPFD; HEL-220; IBMPFD1; HEL-S-70
• NCBI Protein Information: transitional endoplasmic reticulum ATPase
• UniProt Protein Name: Transitional endoplasmic reticulum ATPase
• Protein Family: Teratocarcinoma-derived growth factor
• UniProt Gene Name: VCP
• UniProt Synonym Gene Names: TER ATPase; VCP
• UniProt Entry Name: TERA_HUMAN

NCBI Description

The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq, Jul 2008]

Uniprot Description

VCP: valosin-containing protein (VCP) is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the nuclear envelope and of the transitional endoplasmic reticulum (tER). Regulates NFKappaB pathway, which is important for metastasis of osteosarcoma. Tyrosine phosphorylation regulates its cell cycle-dependent nuclear localization.

Protein type: DNA repair, damage; Hydrolase; Endoplasmic reticulum; EC 3.6.4.6; Chaperone

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: nucleoplasm; proteasome complex; endoplasmic reticulum membrane; intracellular membrane-bound organelle; endoplasmic reticulum; perinuclear region of cytoplasm; cytoplasm; lipid particle; nucleus; cytosol; myelin sheath

Molecular Function: identical protein binding; protein domain specific binding; protein binding; MHC class I protein binding; ubiquitin protein ligase binding; ATPase activity; polyubiquitin binding; ADP binding; lipid binding; protein phosphatase binding; ATP binding

Biological Process: caspase activation; proteasomal ubiquitin-dependent protein catabolic process; ER to Golgi vesicle-mediated transport; ER-associated protein catabolic process; unfolded protein response; protein ubiquitination; viral genome replication; DNA repair; ATP metabolic process; retrograde protein transport, ER to cytosol; regulation of apoptosis; establishment of protein localization; bypass DNA synthesis; positive regulation of protein complex assembly; NADH metabolic process; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; double-strand break repair; positive regulation of protein catabolic process; protein amino acid N-linked glycosylation via asparagine; response to DNA damage stimulus; protein homooligomerization

Disease: Amyotrophic Lateral Sclerosis 14, With Or Without Frontotemporal Dementia; Inclusion Body Myopathy With Early-onset Paget Disease With Or Without Frontotemporal Dementia 1