VCP Polyclonal Antibody | anti-VCP antibody

Anti-VCP Antibody

Cat. Num. AAA178176

• Gene Names: VCP; p97; TERA; ALS14; CMT2Y; IBMPFD; HEL-220; IBMPFD1; HEL-S-70
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: VCP; Polyclonal Antibody; Anti-VCP Antibody; Transitional endoplasmic reticulum ATPase; 15S Mg(2+) ATPase p97 subunit; 15S Mg(2+)-ATPase p97 subunit; ALS14; ATPase p97; IBMPFD; MGC131997; MGC148092; MGC8560; p97; TER ATPase; TERA; TERA_HUMAN; Valosin containing protein; Valosin-containing protein; Yeast Cdc48p homolog antibody; valosin containing protein; anti-VCP antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 806

• Applicable Applications for anti-VCP antibody: Western Blot (WB), Immunohistochemistry (IHC) Formalin/Paraffin
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml; Immunohistochemistry (IHC) Formalin Concentration: 0.5-1ug/ml
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human VCP (732-760aa RRDHFEEAMRFARRSVSDNDIRKYEMFAQ), identical to the related rat and mouse sequences.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- VCP Picoband antibody, MBS178176, Western blottingAll lanes: Anti VCP (MBS178176) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Mouse Brain Tissue Lysate at 50ugLane 3: HELA Whole Cell Lysate at 40ugLane 4: 293T Whole Cell Lysate at 40ugPredicted bind size: 89KDObserved bind size: 89KD )

Immunohistochemistry (IHC)

(Anti- VCP Picoband antibody, MBS178176, IHC(P)IHC(P): Mouse Brain Tissue )

Immunohistochemistry (IHC)

(Anti- VCP Picoband antibody, MBS178176, IHC(P)IHC(P): Rat Brain Tissue )

Immunohistochemistry (IHC)

(Anti- VCP Picoband antibody, MBS178176, IHC(P)IHC(P): Human Glioma Tissue )

Immunohistochemistry (IHC)

(Anti- VCP Picoband antibody, MBS178176, IHC(F)IHC(F): Mouse Brain Tissue )

Immunohistochemistry (IHC)

(Anti- VCP Picoband antibody, MBS178176, IHC(F)IHC(F): Rat Brain Tissue )

Related Product Information for anti-VCP antibody

Description: Rabbit IgG polyclonal antibody for Transitional endoplasmic reticulum ATPase(VCP) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat.

Background: Valosin-containing protein also called CDC48 is an enzyme that in humans is encoded by the VCP gene. It is a member of the AAA+ (ATPase associated with various activities) protein family. The VCP gene maps to chromosome 9p13.3. It is necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. It is involved in the formation of the transitional endoplasmic reticulum. This gene plays a role in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. It also involved in DNA damage response: recruited to double-strand breaks (DSBs) sites in a RNF8- and RNF168-dependent manner and promotes the recruitment of TP53BP1 at DNA damage sites.

References

1. Custer, S. K., Neumann, M., Lu, H., Wright, A. C., Taylor, J. P. Transgenic mice expressing mutant forms VCP recapitulate the full spectrum of IBMPFD including degeneration in muscle, brain and bone. Hum. Molec. Genet. 19: 1741-1755, 2010. 2. Haubenberger, D., Bittner, R. E., Rauch-Shorny, S., Zimprich, F., Mannhalter, C., Wagner, L., Mineva, I., Vass, K., Auff, E., Zimprich, A. Inclusion body myopathy and Paget disease is linked to a novel mutation in the VCP gene. Neurology 65: 1304-1305, 2005. 3. Mueller, B., Klemm, E. J., Spooner, E., Claessen, J. H., Ploegh, H. L. SEL1L nucleates a protein complex required for dislocation of misfolded glycoproteins. Proc. Nat. Acad. Sci. 105: 12325-12330, 2008. 4. Sacconi, S., Camano, P., de Greef, J. C., Lemmers, R. J. L. F., Salviati, L., Boileau, P., Lopez de Munain Arregui, A., van der Maarel, S. M., Desnuelle, C. Patients with a phenotype consistent with facioscapulohumeral muscular dystrophy display genetic and epigenetic heterogeneity. J. Med. Genet. 49: 41-46, 2012.

NCBI and Uniprot Product Information

• NCBI GI #: 6005942
• NCBI GeneID: 7415
• NCBI Accession #: NP_009057.1
• NCBI GenBank Nucleotide #: NM_007126.3
• UniProt Accession #: P55072; Q0V924; Q2TAI5; Q969G7; Q9UCD5; B2R5T8
• Molecular Weight: 89,322 Da
• NCBI Official Full Name: transitional endoplasmic reticulum ATPase
• NCBI Official Synonym Full Names: valosin containing protein
• NCBI Official Symbol: VCP
• NCBI Official Synonym Symbols: p97; TERA; ALS14; CMT2Y; IBMPFD; HEL-220; IBMPFD1; HEL-S-70
• NCBI Protein Information: transitional endoplasmic reticulum ATPase
• UniProt Protein Name: Transitional endoplasmic reticulum ATPase
• Protein Family: VCP-like ATPase
• UniProt Gene Name: VCP
• UniProt Synonym Gene Names: TER ATPase; VCP
• UniProt Entry Name: TERA_HUMAN

NCBI Description

The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq, Jul 2008]

Uniprot Description

VCP: valosin-containing protein (VCP) is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the nuclear envelope and of the transitional endoplasmic reticulum (tER). Regulates NFKappaB pathway, which is important for metastasis of osteosarcoma. Tyrosine phosphorylation regulates its cell cycle-dependent nuclear localization.

Protein type: DNA repair, damage; Hydrolase; Chaperone; EC 3.6.4.6; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; endoplasmic reticulum membrane; intracellular membrane-bound organelle; lipid particle; myelin sheath; nucleoplasm; nucleus; perinuclear region of cytoplasm; proteasome complex

Molecular Function: ADP binding; ATP binding; ATPase activity; identical protein binding; lipid binding; MHC class I protein binding; polyubiquitin binding; protein binding; protein domain specific binding; protein phosphatase binding; ubiquitin protein ligase binding

Biological Process: ATP metabolic process; bypass DNA synthesis; caspase activation; DNA repair; double-strand break repair; ER to Golgi vesicle-mediated transport; ER-associated protein catabolic process; establishment of protein localization; NADH metabolic process; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein catabolic process; positive regulation of protein complex assembly; proteasomal ubiquitin-dependent protein catabolic process; protein amino acid N-linked glycosylation via asparagine; protein homooligomerization; protein ubiquitination; regulation of apoptosis; response to DNA damage stimulus; retrograde protein transport, ER to cytosol; unfolded protein response; viral genome replication

Disease: Amyotrophic Lateral Sclerosis 14, With Or Without Frontotemporal Dementia; Inclusion Body Myopathy With Early-onset Paget Disease With Or Without Frontotemporal Dementia 1