RFK blocking peptide

RFK Antibody (N-term) Blocking Peptide

• Gene Names: RFK; RIFK
• Synonyms: RFK; RFK Antibody (N-term) Blocking Peptide; Riboflavin kinase; ATP:riboflavin 5'-phosphotransferase; Flavokinase; RFK blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of human RFK. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 155

• Cellular Location: Cytoplasm.
• Tissue Location: Detected in brain, placenta and urinary bladder
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for RFK blocking peptide

Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF-activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase.

NCBI and Uniprot Product Information

• NCBI GI #: 209572667
• NCBI GeneID: 55312
• UniProt Accession #: Q969G6; Q5JSG9; Q9NUT7
• Molecular Weight: 17,623 Da
• NCBI Official Full Name: Riboflavin kinase
• NCBI Official Synonym Full Names: riboflavin kinase
• NCBI Official Symbol: RFK
• NCBI Official Synonym Symbols: RIFK
• NCBI Protein Information: riboflavin kinase
• UniProt Protein Name: Riboflavin kinase
• UniProt Gene Name: RFK
• UniProt Entry Name: RIFK_HUMAN

NCBI Description

Riboflavin kinase (RFK; EC 2.7.1.26) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis (Karthikeyan et al., 2003 [PubMed 12623014]).[supplied by OMIM, Nov 2009]

Uniprot Description

RFK: Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN).

Protein type: Kinase, other; EC 2.7.1.26; Cofactor and Vitamin Metabolism - riboflavin

Chromosomal Location of Human Ortholog: 9q21.13

Cellular Component: cytosol

Molecular Function: riboflavin kinase activity

Biological Process: apoptosis; positive regulation of NAD(P)H oxidase activity; riboflavin metabolic process