SDS-PAGE
Flavokinase / RFK Recombinant Protein | RFK recombinant protein
Recombinant Human Flavokinase / RFK Protein (His tag)
Gene Names
RFK; RIFK
Purity
> 90 % as determined by SDS-PAGE
Synonyms
Flavokinase / RFK; Recombinant Human Flavokinase / RFK Protein (His tag); RFK; RFK recombinant protein
Host
E Coli
Purity/Purification
> 90 % as determined by SDS-PAGE
Form/Format
Supplied as sterile 20mM Tris, 10% glycerol, pH 8.0
Sequence Length
155
Application Notes
The recombinant human RFK consists of 170 amino acids and predicts a molecular mass of 19.5 KDa. It migrates as an approximately 20 KDa band in SDS-PAGE under reducing conditions.
Predicted N Terminal
His
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
SDS-PAGE
SDS-PAGE
Related Product Information for RFK recombinant protein
Background: Flavokinase is a member of the transferases family, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. Flavokinase is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis. It has been proposed that TNF, through the activation of the flavokinase gene, enhances the incorporation of FAD in NADPH oxidase enzymes, which is a critical step for the assembly and activation of NADPH oxidase.
Description: A DNA sequence encoding the mature form of human RFK (Q969G6) (Met1-His155) was expressed with a polyhistide tag at the N-terminus.
Description: A DNA sequence encoding the mature form of human RFK (Q969G6) (Met1-His155) was expressed with a polyhistide tag at the N-terminus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,623 Da
NCBI Official Full Name
riboflavin kinase
NCBI Official Synonym Full Names
riboflavin kinase
NCBI Official Symbol
RFK
NCBI Official Synonym Symbols
RIFK
NCBI Protein Information
riboflavin kinase
UniProt Protein Name
Riboflavin kinase
UniProt Gene Name
RFK
NCBI Description
Riboflavin kinase (RFK; EC 2.7.1.26) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis (Karthikeyan et al., 2003 [PubMed 12623014]).[supplied by OMIM, Nov 2009]
Uniprot Description
RFK: Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN).
Protein type: Cofactor and Vitamin Metabolism - riboflavin; EC 2.7.1.26; Kinase, other
Chromosomal Location of Human Ortholog: 9q21.13
Cellular Component: cytosol
Molecular Function: riboflavin kinase activity
Biological Process: apoptosis; positive regulation of NAD(P)H oxidase activity; riboflavin metabolic process
Research Articles on RFK
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Product Notes
The RFK rfk (Catalog #AAA2545028) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The recombinant human RFK consists of 170 amino acids and predicts a molecular mass of 19.5 KDa. It migrates as an approximately 20 KDa band in SDS-PAGE under reducing conditions. Researchers should empirically determine the suitability of the RFK rfk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Flavokinase / RFK, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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