Western Blot (WB)
(Western blot analysis of extracts from HeLa (Line 1, 4, 7 and 10), K562 (Line 2, 5, 8 and 11) and 293 (Line 3, 6, 9 and 12) cells, untreated or treated with PMA (1etaM 30min), using Connexin 43 antibody (Lane 1, 2, 3, 4, 5 and 6) and Connexin 43 (phospho-Ser367) antibody (Lane 7, 8, 9, 10, 11 and 12).)
Rabbit Connexin 43 Polyclonal Antibody | anti-CX43 antibody
Connexin 43 Antibody (Ser367)
Gene Names
GJA1; CX43
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Chromatography
Synonyms
Connexin 43; Polyclonal Antibody; Connexin 43 Antibody (Ser367); Rabbit Polyclonal Connexin 43 Antibody (Ser367); Polyclonal Connexin 43 antibody; Anti-Connexin 43 antibody; connexin43 antibody; Connexin-43 antibody; Cx43 antibody; CXA1 antibody; DFNB38 antibody; Gap junction 43 kDa heart protein antibody; Gap junction alpha-1 protein antibody; gap junction protein alpha 1 43kDa antibody; gap junction protein alpha-like antibody; GJA1 antibody; GJAL antibody; HSS antibody; ODDD antibody; anti-CX43 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
Connexin 43 antibody (Ser367) detects endogenous levels of Connexin 43 only when phosphorylated at serine 367
Purity/Purification
Affinity Chromatography
Form/Format
Supplied in PBS buffer (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol
Concentration
1 mg/ml (varies by lot)
Sequence Length
125
Applicable Applications for anti-CX43 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:100
IHC: 1:50-1:100
Immunogen
Connexin 43 antibody was raised in Rabbit using a synthesized phosphopeptide derived from human Connexin 43 around the phosphorylation site of serine 367 (R-A-SP-S-R) as the immunogen.
Cross-Reactivity
Mouse, Rat
Preparation and Storage
Store at -20 degree C in small aliquots. Avoid repeat freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts from HeLa (Line 1, 4, 7 and 10), K562 (Line 2, 5, 8 and 11) and 293 (Line 3, 6, 9 and 12) cells, untreated or treated with PMA (1etaM 30min), using Connexin 43 antibody (Lane 1, 2, 3, 4, 5 and 6) and Connexin 43 (phospho-Ser367) antibody (Lane 7, 8, 9, 10, 11 and 12).)
Western Blot (WB)
(Western blot analysis of extracts from HeLa (Line 1, 4, 7 and 10), K562 (Line 2, 5, 8 and 11) and 293 (Line 3, 6, 9 and 12) cells, untreated or treated with PMA (1etaM 30min), using Connexin 43 antibody (Lane 1, 2, 3, 4, 5 and 6) and Connexin 43 (phospho-Ser367) antibody (Lane 7, 8, 9, 10, 11 and 12).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Connexin 43 antibody (Ser367))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Connexin 43 antibody (Ser367))
Related Product Information for anti-CX43 antibody
Connexin 43 is an integral membrane protein of the connexin family, alpha-type (group II) subfamily. Hexamers of connexin-43 form connexons, which aggregate together to form gap junctions, through which materials of low MW diffuse from one cell to a neighboring cell.
Product Categories/Family for anti-CX43 antibody
NCBI and Uniprot Product Information
Research Articles on CX43
Similar Products
Product Notes
The CX43 (Catalog #AAA5313676) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Connexin 43 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:100. Researchers should empirically determine the suitability of the CX43 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Connexin 43, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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