Rabbit anti-Human RFK Polyclonal Antibody | anti-Cmaq_1224 antibody

RFK, NT (Riboflavin Kinase, ATP:riboflavin 5'-phosphotransferase, Flavokinase)

Cat. Num. AAA630969

• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: RFK; Polyclonal Antibody; NT (Riboflavin Kinase; ATP:riboflavin 5'-phosphotransferase; Flavokinase); Anti -RFK; anti-Cmaq_1224 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RFK.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-Cmaq_1224 antibody: ELISA (EL/EIA), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the N-terminal region of human RFK (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(Western blot analysis of RFK (arrow) using rabbit polyclonal RFK Antibody (C15) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RFK gene.)

Flow Cytometry (FC/FACS)

(RFK Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-Cmaq_1224 antibody

RFK (riboflavin kinase) catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN).

Product Categories/Family for anti-Cmaq_1224 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 172047999
• NCBI GeneID: 5710113
• UniProt Accession #: A8ME45
• Molecular Weight: 25,887 Da
• NCBI Official Full Name: Riboflavin kinase
• NCBI Official Symbol: Cmaq_1224
• NCBI Protein Information: hypothetical protein
• UniProt Protein Name: Riboflavin kinase
• UniProt Gene Name: ribK
• UniProt Synonym Gene Names: RFK
• UniProt Entry Name: RIFK_CALMQ

Uniprot Description

Function: Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN)

By similarity. HAMAP-Rule MF_01285

Catalytic activity: CTP + riboflavin = CDP + FMN. HAMAP-Rule MF_01285

Cofactor: Binds 1 magnesium ion per subunit

By similarity. HAMAP-Rule MF_01285

Pathway: Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP-Rule MF_01285

Sequence similarities: Belongs to the archaeal riboflavin kinase family.