Typical Testing Data/Standard Curve (for reference only)
Chicken Progonadoliberin-1 (GNRH1) ELISA Kit | GNRH1 elisa kit
Chicken Progonadoliberin-1 (GNRH1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: GNRH1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-GNRH1 antibody and an GNRH1-HRP conjugate. The assay sample and buffer are incubated together with GNRH1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GNRH1 concentration since GNRH1 from samples and GNRH1-HRP conjugate compete for the anti-GNRH1 antibody binding site. Since the number of sites is limited, as more sites are occupied by GNRH1 from the sample, fewer sites are left to bind GNRH1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GNRH1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is secreted and then cleaved to form the 10 aa luteinizing hormone-releasing hormone (LHRH, also known as gonadoliberin-1), and prolactin release-inhibiting factor (also known as GnRH-associated peptide 1). LHRH stimulates the release of luteinizing and follicle stimulating hormones, which are important for reproduction. Mutation in this gene are associated with hypogonadotropic hypogonadism. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jul 2012]
Uniprot Description
GNRH1: Stimulates the secretion of gonadotropins; it stimulates the secretion of both luteinizing and follicle-stimulating hormones. Belongs to the GnRH family.
Protein type: Secreted; Cell cycle regulation; Hormone; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 8p21-p11.2
Cellular Component: extracellular space; extracellular region
Molecular Function: hormone activity; luteinizing hormone-releasing factor activity
Biological Process: negative regulation of cell proliferation; response to ethanol; ovulation cycle; cell-cell signaling; regulation of gene expression; multicellular organismal development; female pregnancy; signal transduction; response to corticosteroid stimulus; negative regulation of apoptosis
Disease: Hypogonadotropic Hypogonadism 7 With Or Without Anosmia
Research Articles on GNRH1
Similar Products
Product Notes
The Chicken GNRH1 gnrh1 (Catalog #AAA7234632) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7234632 ELISA Kit recognizes Chicken GNRH1. It is sometimes possible for the material contained within the vial of "Progonadoliberin-1 (GNRH1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
