DENND1B blocking peptide

DENND1B Antibody (N-term)

• Gene Names: DENND1B; FAM31B; C1ORF18; C1orf218
• Synonyms: DENND1B; DENND1B Antibody (N-term); DENN domain-containing protein 1B; Connecdenn 2; Protein FAM31B; C1orf218; FAM31B; DENND1B blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 76

• Cellular Location: Cytoplasm, cytosol. Cytoplasmic vesicle, clathrin-coated vesicle
• Tissue Location: Highly expressed in dendritic and natural killer cells and at lower levels in other myeloid lineage cells and in pituitary. Significantly up-regulated in effector memory T- cells as compared with naive T-cells
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for DENND1B blocking peptide

Guanine nucleotide exchange factor (GEF) for RAB35. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB35 into its active GTP-bound form. May play a role in clathrin- mediated endocytosis.

NCBI and Uniprot Product Information

• NCBI GI #: 304571967
• NCBI GeneID: 163486
• NCBI Accession #: NP_001182145.1
• NCBI GenBank Nucleotide #: NM_001195216.1
• UniProt Accession #: Q6P3S1; Q5T3B8; Q5T3B9; Q5T3C1; Q5TAI8; Q6B0I8; Q8NDT1; Q8TBE6; Q9H774; Q9NXU2; B5MD89; D3PFD5
• Molecular Weight: 48,260 Da
• NCBI Official Full Name: DENN domain-containing protein 1B isoform 4
• NCBI Official Synonym Full Names: DENN domain containing 1B
• NCBI Official Symbol: DENND1B
• NCBI Official Synonym Symbols: FAM31B; C1ORF18; C1orf218
• NCBI Protein Information: DENN domain-containing protein 1B
• UniProt Protein Name: DENN domain-containing protein 1B
• Protein Family: DENN domain-containing protein
• UniProt Gene Name: DENND1B
• UniProt Entry Name: DEN1B_HUMAN

NCBI Description

Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).[supplied by OMIM, Nov 2010]

Uniprot Description

Guanine nucleotide exchange factor (GEF) for RAB35 that acts as a regulator of T-cell receptor (TCR) internalization in TH2 cells (PubMed:20154091, PubMed:20937701, PubMed:24520163, PubMed:26774822). Acts by promoting the exchange of GDP to GTP, converting inactive GDP-bound RAB35 into its active GTP-bound form (PubMed:20154091, PubMed:20937701). Plays a role in clathrin-mediated endocytosis (PubMed:20154091). Controls cytokine production in TH2 lymphocytes by controlling the rate of TCR internalization and routing to endosomes: acts by mediating clathrin-mediated endocytosis of TCR via its interaction with the adapter protein complex 2 (AP-2) and GEF activity (PubMed:26774822). Dysregulation leads to impaired TCR down-modulation and recycling, affecting cytokine production in TH2 cells (PubMed:26774822).