Rabbit anti-Human DENND1B Polyclonal Antibody | anti-DENND1B antibody

DENND1B, NT (DENND1B, C1orf218, FAM31B, DENN domain-containing protein 1B, Connecdenn 2, Protein FAM31B)

Cat. Num. AAA6012170

• Gene Names: DENND1B; FAM31B; C1ORF18; C1orf218; RP11-53I24.2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: DENND1B; Polyclonal Antibody; NT (DENND1B; C1orf218; FAM31B; DENN domain-containing protein 1B; Connecdenn 2; Protein FAM31B); Anti -DENND1B; anti-DENND1B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-DENND1B antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: DENND1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-76 amino acids from the N-terminal region of human DENND1B.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DENND1B Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the DENND1B antibody detected the DENND1B protein (arrow).)

Immunohistochemistry (IHC)

(DENND1B antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DENND1B antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(DENND1B Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-DENND1B antibody

Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).

Product Categories/Family for anti-DENND1B antibody

Antibodies; Abs to KIAA; orf

NCBI and Uniprot Product Information

• NCBI GI #: 304571967
• NCBI GeneID: 163486
• NCBI Accession #: NP_001182145.1
• NCBI GenBank Nucleotide #: NM_001195216.1
• UniProt Accession #: Q6P3S1; Q5T3B8; Q5T3B9; Q5T3C1; Q5TAI8; Q6B0I8; Q8NDT1; Q8TBE6; Q9H774; Q9NXU2; B5MD89; D3PFD5
• Molecular Weight: 48,260 Da
• NCBI Official Full Name: DENN domain-containing protein 1B isoform 4
• NCBI Official Synonym Full Names: DENN/MADD domain containing 1B
• NCBI Official Symbol: DENND1B
• NCBI Official Synonym Symbols: FAM31B; C1ORF18; C1orf218; RP11-53I24.2
• NCBI Protein Information: DENN domain-containing protein 1B; connecdenn 2; family with sequence similarity 31, member B
• UniProt Protein Name: DENN domain-containing protein 1B
• Protein Family: DENN domain-containing protein
• UniProt Gene Name: DENND1B
• UniProt Synonym Gene Names: C1orf218; FAM31B
• UniProt Entry Name: DEN1B_HUMAN

NCBI Description

Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).[supplied by OMIM, Nov 2010]

Uniprot Description

DENND1B: a guanine nucleotide exchange factor (GEF) regulating clathrin-mediated endocytosis through RAB35 activation. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB35 into its active GTP-bound form. Interacts with RAB35. Interacts with clathrin heavy chain/CLTC and with AP2A2. Highly expressed in dendritic and natural killer cells and at lower levels in other myeloid lineage cells and in pituitary. Significantly up-regulated in effector memory T-cells as compared with naive T-cells. Five isoforms of the human protein are produced by alternative splicing. Isoform 5 interacts with AP2B1.

Protein type: GEFs, Rab; GEFs

Chromosomal Location of Human Ortholog: 1q31.3

Cellular Component: clathrin-coated vesicle; cytosol

Molecular Function: Rab guanyl-nucleotide exchange factor activity

Biological Process: protein transport