Rabbit anti-Human DENND1B Polyclonal Antibody | anti-DENND1B antibody

DENND1B, NT (DENND1B, C1orf218, FAM31B, DENN domain-containing protein 1B, Connecdenn 2, Protein FAM31B) (Biotin)

Cat. Num. AAA6291708

• Gene Names: DENND1B; FAM31B; C1ORF18; C1orf218
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DENND1B; Polyclonal Antibody; NT (DENND1B; C1orf218; FAM31B; DENN domain-containing protein 1B; Connecdenn 2; Protein FAM31B) (Biotin); Protein FAM31B; anti-DENND1B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-DENND1B antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: DENND1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-76 amino acids from the N-terminal region of human DENND1B.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DENND1B Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the DENND1B antibody detected the DENND1B protein (arrow).)

Immunohistochemistry (IHC)

(DENND1B antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DENND1B antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(DENND1B Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-DENND1B antibody

Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).

Product Categories/Family for anti-DENND1B antibody

Antibodies; KIAA; orf

References

Marat, A.L., et al. J. Biol. Chem. 285(14):10627-10637(2010). Sleiman, P.M., et al. N. Engl. J. Med. 362(1):36-44(2010). Venter, J.C., et al. Science 291(5507):1304-1351(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 304571965
• NCBI GeneID: 163486
• NCBI Accession #: NP_001182144.1; NP_001182145.1
• NCBI GenBank Nucleotide #: NM_001195215.1
• UniProt Accession #: Q6P3S1; Q5T3B8; Q5T3B9; Q5T3C1; Q5TAI8; Q6B0I8; Q8NDT1; Q8TBE6; Q9H774; Q9NXU2; B5MD89; D3PFD5
• Molecular Weight: 86,552 Da
• NCBI Official Full Name: DENN domain-containing protein 1B isoform 3
• NCBI Official Synonym Full Names: DENN/MADD domain containing 1B
• NCBI Official Symbol: DENND1B
• NCBI Official Synonym Symbols: FAM31B; C1ORF18; C1orf218
• NCBI Protein Information: DENN domain-containing protein 1B
• UniProt Protein Name: DENN domain-containing protein 1B
• Protein Family: DENN domain-containing protein
• UniProt Gene Name: DENND1B
• UniProt Synonym Gene Names: C1orf218; FAM31B
• UniProt Entry Name: DEN1B_HUMAN

NCBI Description

Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).[supplied by OMIM, Nov 2010]