SHP-1 Cell Lysate | PTPN6 cell lysate
Mouse SHP-1 Baculovirus-Insect cells Overexpression Lysate
Gene Names
Ptpn6; me; hcp; Hcph; Ptp1C; SHP-1; 70Z-SHP; SH-PTP1; PTPTY-42
Applications
Western Blot
Synonyms
SHP-1; Mouse SHP-1 Baculovirus-Insect cells Overexpression Lysate; Mouse PTPN6 (aa 207-597) Insect Cell Lysate (WB positive control); protein tyrosine phosphatase; non-receptor type 6; Mouse 70Z-SHP Overexpression Lysate; Mouse hcp Overexpression Lysate; Mouse Hcph Overexpression Lysate; Mouse me Overexpression Lysate; Mouse Ptp1C Overexpression Lysate; Mouse PTPTY-42 Overexpression Lysate; Mouse SH-PTP1 Overexpression Lysate; Mouse SHP-1 Overexpression Lysate; PTPN6 cell lysate
Host
Baculovirus-Insect cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
628aa
Sequence
Ala207-Lys597
Applicable Applications for PTPN6 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse PTPN6 (P29351-2) (Ala207-Lys597) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for PTPN6 cell lysate
PTPN6 is an enzyme that belongs to the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of PTPN6 contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of PTPN6 with its substrates. PTPN6 is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. It has been shown that PTPN6 interacts with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling.
This Mouse SHP-1 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of SHP-1 protein from the overexpression lysate was verified.
This Mouse SHP-1 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of SHP-1 protein from the overexpression lysate was verified.
Product Categories/Family for PTPN6 cell lysate
References
Yu CL, et al. (2000) Cytosolic tyrosine dephosphorylation of STAT5. Potential role of SHP-2 in STAT5 regulation. J Biol Chem. 275(1):599-604. Wu DW, et al. (2000) SH2-Containing protein tyrosine phosphatase-1 (SHP-1) association with Jak2 in UT-7/Epo cells. Blood Cells Mol Dis. 26(1):15-24. Jiao H, et al. (1996) Direct association with and dephosphorylation of Jak2 kinase by the SH2-domain-containing protein tyrosine phosphatase SHP-1. Mol Cell Biol. 16(12):6985-92.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
67,559 Da
NCBI Official Full Name
tyrosine-protein phosphatase non-receptor type 6 isoform b
NCBI Official Synonym Full Names
protein tyrosine phosphatase, non-receptor type 6
NCBI Official Symbol
Ptpn6
NCBI Official Synonym Symbols
me; hcp; Hcph; Ptp1C; SHP-1; 70Z-SHP; SH-PTP1; PTPTY-42
NCBI Protein Information
tyrosine-protein phosphatase non-receptor type 6; motheaten; protein-tyrosine phosphatase 1C; hematopoietic cell protein-tyrosine phosphatase
UniProt Protein Name
Tyrosine-protein phosphatase non-receptor type 6
Protein Family
UniProt Gene Name
Ptpn6
UniProt Synonym Gene Names
Hcp; Hcph; Ptp1C; PTP-1C; SHP-1
UniProt Entry Name
PTN6_MOUSE
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Product Notes
The PTPN6 ptpn6 (Catalog #AAA8116917) is a Cell Lysate produced from Baculovirus-Insect cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 628aa. AAA Biotech's SHP-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the PTPN6 ptpn6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Ala207-Lys 597. It is sometimes possible for the material contained within the vial of "SHP-1, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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