Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
KIF5B-ALK recombinant protein
KIF5B-ALK (Kex24Aex20), Active
Applications
Kinase Assay
Synonyms
KIF5B-ALK; KIF5B-ALK (Kex24Aex20); Active; KIF5B: KINH; UKHC; KNS ALK: ALK (Ki-1); CD246; KIF5B-ALK recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, and 25% glycerol.
Applicable Applications for KIF5B-ALK recombinant protein
Kinase Assay
Tag
GST
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for KIF5B-ALK recombinant protein
Recombinant human fusion KIF5B (1-921a.a., exons1-24) -ALK (1058a.a.-end, exons20-29) protein was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number of KIF5B is NM_004521 and ALK is NM_004304.
Anaplastic large cell lymphomas are associated with chromosomal aberrations involving the anaplastic lymphoma kinase (ALK) gene, resulting in the expression of novel chimeric ALK proteins with transforming properties. Many chromosomal rearrangements leading to enhanced ALK activity have been described and are implicated in a number of cancer types (1). A fusion protein of kinesin family member 5B (KIF5B) exon 15 to ALK exon 20 has been reported in a primary lung adenocarcinoma (2). Overexpression of KIF5B-ALK increased cell proliferation, migration and invation via activating the STAT3 and PKB (3).
Anaplastic large cell lymphomas are associated with chromosomal aberrations involving the anaplastic lymphoma kinase (ALK) gene, resulting in the expression of novel chimeric ALK proteins with transforming properties. Many chromosomal rearrangements leading to enhanced ALK activity have been described and are implicated in a number of cancer types (1). A fusion protein of kinesin family member 5B (KIF5B) exon 15 to ALK exon 20 has been reported in a primary lung adenocarcinoma (2). Overexpression of KIF5B-ALK increased cell proliferation, migration and invation via activating the STAT3 and PKB (3).
Product Categories/Family for KIF5B-ALK recombinant protein
References
1. Palmer R H, et al: Anaplastic lymphoma kinase: signalling in development and disease. Biochem J. 2009 May 27;420(3):345-61.
2. Takeuchi K, et al: KIF5B-ALK, a novel fusion oncokinase identified by an immunohistochemistry-based diagnostic system for ALK-positive lung cancer. 2009 May 1;15(9):3143-9.
3. Wong DW, et al: A novel KIF5B-ALK variant in nonsmall cell lung cancer. Cancer. 2011 Jun 15;117(12):2709-18.
2. Takeuchi K, et al: KIF5B-ALK, a novel fusion oncokinase identified by an immunohistochemistry-based diagnostic system for ALK-positive lung cancer. 2009 May 1;15(9):3143-9.
3. Wong DW, et al: A novel KIF5B-ALK variant in nonsmall cell lung cancer. Cancer. 2011 Jun 15;117(12):2709-18.
NCBI and Uniprot Product Information
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Product Notes
The KIF5B-ALK (Catalog #AAA516556) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's KIF5B-ALK can be used in a range of immunoassay formats including, but not limited to, Kinase Assay. Researchers should empirically determine the suitability of the KIF5B-ALK for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KIF5B-ALK, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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