Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Transmembrane protein 158 (TMEM158) Standard Curve.)
Human Transmembrane Protein 158 (TMEM158) ELISA Kit | TMEM158 elisa kit
Human Transmembrane Protein 158 (TMEM158) ELISA Kit
Gene Names
TMEM158; BBP; RIS1; p40BBP
Reactivity
Human
Synonyms
Transmembrane Protein 158 (TMEM158); Human Transmembrane Protein 158 (TMEM158) ELISA Kit; TMEM158; BBP; DKFZp586E1621; RIS1; p40BBP; BINP receptor; Ras induced senescence 1; Ras-induced senescence 1; brain injury-derived neurotrophic peptide (BINP) binding protein; brain specific binding protein; TMEM158 elisa kit
Reactivity
Human
Specificity
Human Transmembrane protein 158 (TMEM158) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMEM158. No significant cross-reactivity or interference between Human TMEM158 and analogues was observed.
Sequence Length
286
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Transmembrane protein 158 (TMEM158) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Transmembrane protein 158 (TMEM158) Standard Curve.)
Related Product Information for TMEM158 elisa kit
Description: This Human Transmembrane protein 158 (TMEM158) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM158 in samples. An antibody specific for TMEM158 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TMEM158 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM158 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM158 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Constitutive activation of the Ras pathway triggers an irreversible proliferation arrest reminiscent of replicative senescence. Transcription of this gene is upregulated in response to activation of the Ras pathway, but not under other conditions that induce senescence. RIS1 is similar to a rat cell surface receptor proposed to function in a neuronal survival pathway. RIS1 is not upregulated in association to any of the above-mentioned processes, but exclusively during Ras-senescence. Furthermore, RIS1 is also upregulated by the transcriptional factor Ets2, which is a known mediator of Ras-induced senescence. Interestingly, RIS1 is located at chromosomal position 3p21.3 and, more specifically, it is included in a short segment of just 1 Mb previously defined by other investigators for its tumor-suppressor activity.
Background: Constitutive activation of the Ras pathway triggers an irreversible proliferation arrest reminiscent of replicative senescence. Transcription of this gene is upregulated in response to activation of the Ras pathway, but not under other conditions that induce senescence. RIS1 is similar to a rat cell surface receptor proposed to function in a neuronal survival pathway. RIS1 is not upregulated in association to any of the above-mentioned processes, but exclusively during Ras-senescence. Furthermore, RIS1 is also upregulated by the transcriptional factor Ets2, which is a known mediator of Ras-induced senescence. Interestingly, RIS1 is located at chromosomal position 3p21.3 and, more specifically, it is included in a short segment of just 1 Mb previously defined by other investigators for its tumor-suppressor activity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
TMEM158 protein
NCBI Official Synonym Full Names
transmembrane protein 158 (gene/pseudogene)
NCBI Official Symbol
TMEM158
NCBI Official Synonym Symbols
BBP; RIS1; p40BBP
NCBI Protein Information
transmembrane protein 158
UniProt Protein Name
Transmembrane protein 158
Protein Family
UniProt Gene Name
TMEM158
UniProt Synonym Gene Names
HBBP; RIS1; p40BBP
UniProt Entry Name
TM158_HUMAN
NCBI Description
Constitutive activation of the Ras pathway triggers an irreversible proliferation arrest reminiscent of replicative senescence. Transcription of this gene is upregulated in response to activation of the Ras pathway, but not under other conditions that induce senescence. The encoded protein is similar to a rat cell surface receptor proposed to function in a neuronal survival pathway. An allelic polymorphism in this gene results in both functional and non-functional (frameshifted) alleles; the reference genome represents the functional allele. [provided by RefSeq, Jul 2015]
Uniprot Description
Function: Receptor for brain injury-derived neurotrophic peptide (BINP), a synthetic 13-mer peptide
By similarity.
Subcellular location: Membrane; Multi-pass membrane protein
Potential.
Induction: Up-regulated during Ras-induced senesence. Ref.1
Post-translational modification: N-glycosylated
By similarity.
Sequence similarities: Belongs to the TMEM158 family.
Research Articles on TMEM158
Similar Products
Product Notes
The Human TMEM158 tmem158 (Catalog #AAA9713962) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713962 ELISA Kit recognizes Human TMEM158. It is sometimes possible for the material contained within the vial of "Transmembrane Protein 158 (TMEM158), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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