Rabbit PTPN6 Polyclonal Antibody | anti-PTPN6 antibody

PTPN6 Antibody

Cat. Num. AAA2519242

• Gene Names: Ptpn6; me; hcp; Hcph; Ptp1C; SHP-1; 70Z-SHP; SH-PTP1; PTPTY-42
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antigen affinity purification
• Synonyms: PTPN6; Polyclonal Antibody; PTPN6 Antibody; HCP; HCPH; HPTP1C; PTP-1C; SH-PTP1; SHP-1; SHP-1L; SHP1; anti-PTPN6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 595

• Applicable Applications for anti-PTPN6 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Immunogen: Recombinant protein of human PTPN6
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-30810 / sc-287 / sc-33162
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using PTPN6 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human esophageal cancer using PTPN6 antibody at dilution of 1:200 (400x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat brain using PTPN6 antibody at dilution of 1:200 (400x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cell using PTPN6 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-PTPN6 antibody

SHP-1 (PTPN6) is a non-receptor protein tyrosine phosphatase that is expressed primarily in hematopoietic cells. The enzyme is composed of two SH2 domains, a tyrosine phosphatase catalytic domain, and a carboxy-terminal regulatory domain (1). SHP-1 removes phosphates from target proteins to downregulate several tyrosine kinase-regulated pathways. In hematopoietic cells, the amino-terminal SH2 domain of SHP-1 binds to tyrosine phosphorylated erythropoietin receptors (EpoR) to negatively regulate hematopoietic growth (2). Overexpression of SHP-1 in epithelial cells results in dephosphorylation of the Ros receptor tyrosine kinase and subsequent downregulation of Ros-dependent cell proliferation and transformation (3). Following ligand binding in myeloid cells, SHP-1 associates with the IL-3R beta chain and downregulates IL-3-induced tyrosine phosphorylation and cell proliferation (4). Because SHP-1 downregulates various proliferation pathways, SHP-1 is considered a potential tumor suppressor and angiogenesis regulator (5, 6).

NCBI and Uniprot Product Information

• NCBI GI #: 2289910
• NCBI GeneID: 15170
• Molecular Weight: 63,179 Da
• NCBI Official Full Name: PTPN6
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, non-receptor type 6
• NCBI Official Symbol: Ptpn6
• NCBI Official Synonym Symbols: me; hcp; Hcph; Ptp1C; SHP-1; 70Z-SHP; SH-PTP1; PTPTY-42
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 6; hematopoietic cell protein-tyrosine phosphatase; motheaten; protein-tyrosine phosphatase 1C
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 6
• Protein Family: Tyrosine-protein phosphatase non-receptor
• UniProt Gene Name: Ptpn6
• UniProt Synonym Gene Names: Hcp; Hcph; Ptp1C; PTP-1C; SHP-1
• UniProt Entry Name: PTN6_MOUSE

Uniprot Description

SHP-1: an SH2-containing ubiquitously expressed tyrosine-specific protein phosphatase. Plays a key role in hematopoiesis. Directly links growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Three alternatively spliced isoforms have been described.

Protein type: EC 3.1.3.48; Motility/polarity/chemotaxis; Protein phosphatase, tyrosine (non-receptor)

Cellular Component: cytoplasm; nucleolus; alpha-beta T cell receptor complex; intercellular junction; intracellular; nucleus

Molecular Function: protein binding; hydrolase activity; phosphotyrosine binding; phosphoric monoester hydrolase activity; transmembrane receptor protein tyrosine phosphatase activity; protein tyrosine phosphatase activity; SH2 domain binding; phosphoprotein phosphatase activity; protein kinase binding; SH3 domain binding; natural killer cell lectin-like receptor binding

Biological Process: negative regulation of peptidyl-tyrosine phosphorylation; negative regulation of MAP kinase activity; peptidyl-tyrosine phosphorylation; natural killer cell mediated cytotoxicity; negative regulation of MAPKKK cascade; cytokine and chemokine mediated signaling pathway; negative regulation of T cell receptor signaling pathway; positive regulation of cell adhesion mediated by integrin; protein amino acid dephosphorylation; signal transduction; regulation of release of sequestered calcium ion into cytosol; negative regulation of humoral immune response mediated by circulating immunoglobulin; positive regulation of phosphoinositide 3-kinase cascade; cell proliferation; B cell receptor signaling pathway; negative regulation of T cell proliferation; dephosphorylation; negative regulation of B cell receptor signaling pathway; positive regulation of cell proliferation; regulation of B cell differentiation; hemopoietic progenitor cell differentiation; abortive mitotic cell cycle; platelet formation; cell differentiation