EGFL7 cell lysate
Mouse EGFL7 Baculovirus-Insect cells Overexpression Lysate
Gene Names
Egfl7; Zneu1; VE-statin
Applications
Western Blot
Synonyms
EGFL7; Mouse EGFL7 Baculovirus-Insect cells Overexpression Lysate; Mouse EGFL7/VE-statin Insect Cell Lysate (WB positive control); EGF-like-domain; multiple 7; Mouse VE-statin Overexpression Lysate; Mouse Zneu1 Overexpression Lysate; EGFL7 cell lysate
Host
Baculovirus-Insect cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
273aa
Sequence
Glu22-Leu275
Applicable Applications for EGFL7 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse EGFL7 isoform 1 (Q9QXT5-1) (Glu 22-Leu 275) was fused with a polyhistidine tag at the N-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for EGFL7 cell lysate
Epidermal growth factor-like protein 7, also known as EGF-like protein 7, Multiple epidermal growth factor-like domains protein 7, Multiple EGF-like domains protein 7, Vascular endothelial statin, NOTCH4-like protein and EGFL7, is a secreted protein which contains twoEGF-like domains and oneEMI domain. EGFL7 was identified by a number of groups as a putative secreted factor produced by the vascular endothelial cells (ECs). EGFL7 is described as a novel endothelial cell-derived factor involved in the regulation of the spatial arrangement of cells during vascular tube assembly. With its impact on tubulogenesis and vessel shape EGFL7 joined the large family of molecules governing blood vessel formation. EGFL7 regulates midline angioblast migration in zebrafish embryos-a key step in vascular tubulogenesis. EGFL7 is tightly associated with the extracellular matrix (ECM), and it supports EC migration either as a single factor or in combination with other ECM molecules. EGFL7 provides a proper microenvironment for endothelial cell migration, thereby enabling accurate patterning. Our study indicates that the molecular composition of the ECM influences vascular morphogenesis. EGFL7 also regulates vascular tubulogenesis. It inhibits platelet-derived growth factor (PDGF)-BB-induced smooth muscle cell migration and promotes endothelial cells adhesion to the substrate.
This Mouse EGFL7 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of EGFL7 protein from the overexpression lysate was verified.
This Mouse EGFL7 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of EGFL7 protein from the overexpression lysate was verified.
References
Fitch, M.J. et al., 2004, Dev Dyn. 230 (2):316-24. Schmidt, M. et al., 2007, Novartis Found Symp. 283 :18-28. Campagnolo, L.et al., 2008, Gene Expr Patterns 8 (6):389-96. Picuric, S. et al., 2009, Protein Expr Purif. 68 (1):1-6. Nikolic, I. et al., 2010, J Angiogenes Res. 2 (1): 9.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,305 Da
NCBI Official Full Name
epidermal growth factor-like protein 7 isoform 1
NCBI Official Synonym Full Names
EGF-like domain 7
NCBI Official Symbol
Egfl7
NCBI Official Synonym Symbols
Zneu1; VE-statin
NCBI Protein Information
epidermal growth factor-like protein 7
UniProt Protein Name
Epidermal growth factor-like protein 7
Protein Family
UniProt Gene Name
Egfl7
UniProt Synonym Gene Names
Megf7; EGF-like protein 7; Multiple EGF-like domains protein 7; VE-statin
UniProt Entry Name
EGFL7_MOUSE
Uniprot Description
EGFL7: Regulates vascular tubulogenesis in vivo. Inhibits platelet-derived growth factor (PDGF)-BB-induced smooth muscle cell migration and promotes endothelial cells adhesion to the substrate in vitro.
Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis
Cellular Component: extracellular matrix; extracellular region; extracellular space
Molecular Function: calcium ion binding; Notch binding
Biological Process: angiogenesis; cell adhesion; cell differentiation; multicellular organismal development; negative regulation of cell migration; negative regulation of Notch signaling pathway; positive regulation of endothelial cell proliferation; regulation of cell migration
Research Articles on EGFL7
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Product Notes
The EGFL7 egfl7 (Catalog #AAA8116631) is a Cell Lysate produced from Baculovirus-Insect cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 273aa. AAA Biotech's EGFL7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the EGFL7 egfl7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Glu22-Leu2 75. It is sometimes possible for the material contained within the vial of "EGFL7, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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