EGFL7 recombinant protein

EGFL7 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122766

• Gene Names: Egfl7; Zneu1; VE-statin
• Purity: >95% as determined by SDS-PAGE
• Synonyms: EGFL7; EGFL7 Protein; Mouse; Recombinant (His Tag); Mouse EGFL7/VE-statin Protein (His Tag); VE-statin Protein; Zneu1 Protein; EGF-like-domain; multiple 7; EGFL7 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 10% gly, pH7.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Glu22-Leu275

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mouse EGFL7 isoform 1 (Q9QXT5-1)(Glu 22-Leu 275) was fused with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for EGFL7 recombinant protein

Background: Epidermal growth factor-like protein 7, also known as EGF-like protein 7, Multiple epidermal growth factor-like domains protein 7, Multiple EGF-like domains protein 7, Vascular endothelial statin, NOTCH4-like protein and EGFL7, is a secreted protein which contains twoEGF-like domains and oneEMI domain. EGFL7 was identified by a number of groups as a putative secreted factor produced by the vascular endothelial cells (ECs). EGFL7 is described as a novel endothelial cell-derived factor involved in the regulation of the spatial arrangement of cells during vascular tube assembly. With its impact on tubulogenesis and vessel shape EGFL7 joined the large family of molecules governing blood vessel formation. EGFL7 regulates midline angioblast migration in zebrafish embryos-a key step in vascular tubulogenesis. EGFL7 is tightly associated with the extracellular matrix (ECM), and it supports EC migration either as a single factor or in combination with other ECM molecules. EGFL7 provides a proper microenvironment for endothelial cell migration, thereby enabling accurate patterning. Our study indicates that the molecular composition of the ECM influences vascular morphogenesis. EGFL7 also regulates vascular tubulogenesis. It inhibits platelet-derived growth factor (PDGF)-BB-induced smooth muscle cell migration and promotes endothelial cells adhesion to the substrate.

References

Fitch,M.J. et al., 2004, Dev Dyn. 230 (2):316-24. Schmidt,M. et al., 2007, Novartis Found Symp. 283 :18-28. Campagnolo,L.et al., 2008, Gene Expr Patterns 8 (6):389-96. Picuric,S. et al., 2009, Protein Expr Purif. 68 (1):1-6. Nikolic,I. et al., 2010, J Angiogenes Res. 2 (1): 9.

NCBI and Uniprot Product Information

• NCBI GI #: 257096020
• NCBI GeneID: 353156
• NCBI Accession #: NP_001158036.1
• NCBI GenBank Nucleotide #: NM_001164564.1
• UniProt Accession #: Q9QXT5; Q6XD35; Q9DCP5
• Molecular Weight: 28,305 Da
• NCBI Official Full Name: epidermal growth factor-like protein 7 isoform 1
• NCBI Official Synonym Full Names: EGF-like domain 7
• NCBI Official Symbol: Egfl7
• NCBI Official Synonym Symbols: Zneu1; VE-statin
• NCBI Protein Information: epidermal growth factor-like protein 7
• UniProt Protein Name: Epidermal growth factor-like protein 7
• Protein Family: Epidermal growth factor-like protein
• UniProt Gene Name: Egfl7
• UniProt Synonym Gene Names: Megf7; EGF-like protein 7; Multiple EGF-like domains protein 7; VE-statin
• UniProt Entry Name: EGFL7_MOUSE

Uniprot Description

EGFL7: Regulates vascular tubulogenesis in vivo. Inhibits platelet-derived growth factor (PDGF)-BB-induced smooth muscle cell migration and promotes endothelial cells adhesion to the substrate in vitro.

Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis

Cellular Component: extracellular matrix; extracellular region; extracellular space

Molecular Function: calcium ion binding; Notch binding

Biological Process: angiogenesis; cell adhesion; cell differentiation; multicellular organismal development; negative regulation of cell migration; negative regulation of Notch signaling pathway; positive regulation of endothelial cell proliferation; regulation of cell migration