VE-Statin/EGFL-7 recombinant protein

Mouse VE-Statin/EGFL7

Cat. Num. AAA691586

• Gene Names: Egfl7; Zneu1; VE-statin
• Reactivity: Mouse
• Purity: > 95% by SDS-PAGE & silver stain
• Synonyms: VE-Statin/EGFL-7; Mouse VE-Statin/EGFL7; Recombinant Mouse VE-Statin/EGFL7; EGF-like protein 7; Vascular endothelial statin; Multiple EGF-like domains protein 7; VE-Statin/EGFL-7 recombinant protein

• Host: Insect Cells
• Reactivity: Mouse
• Purity/Purification: > 95% by SDS-PAGE & silver stain
• Form/Format: Lyophilized
• Sequence Length: 263

• Buffer: Water
• Preparation and Storage: Lyophilized samples are stable for greater than six months at -20 degree C to -70 degree C. Reconstituted EGFL7 should be stored in working aliquots at -20 degree C. Avoid repeated freeze-thaw cycles!

Testing Data

Related Product Information for VE-Statin/EGFL-7 recombinant protein

EGFL7 is an ~ 30 kDa secreted protein that contain an Emilin-like (EMI) domain (a multimerization motif), and 2 epidermal growth factor (EGF) domains, one of which binds calcium. Based on these domains, it has been hypothesized that EGFL7 may self-assemble like extracellular matrix (ECM) proteins and, thus, could incorporate into ECM. EGFL7 has been reported to stimulate cell adhesion as well as motility in a manner similar to ECM proteins. EGFL7 has been shown to be primarily expressed by developing ECs but also by primordial germ cells and some central nervous system neurons. Interestingly, EGFL7 expression markedly decreases in ECs in postnatal life, but can be strongly up-regulated after various tissue injuries that lead to increased angiogenic responses.

Product Categories/Family for VE-Statin/EGFL-7 recombinant protein

Cytokines & Growth Factors

References

Schmidt MH et al, Nat Cell Biol 11:873, 2009 2. Nikolic et al, Blood 121:3041, 2013 3. Schmidt MH et al, Development 134: 2913-2923 (2007) 4. Socin F et al, EMBO Journal 22: 5700-5711 (2003) 5. Picuric S et al, Protein Expression and Purification 68: 1-6 (2009) 6. Caetano B et al, Protein Expression and Purification 46: 136-142 (2006) 7. Nichol D & Stuhlmann H, Blood 119:1345-1352 (2012)

NCBI and Uniprot Product Information

• NCBI GI #: 257096020
• NCBI GeneID: 353156
• NCBI Accession #: NP_942018.2
• NCBI GenBank Nucleotide #: NM_198725.2
• UniProt Accession #: Q9QXT5; Q6XD35; Q9DCP5
• Molecular Weight: 29,765 Da
• NCBI Official Full Name: epidermal growth factor-like protein 7 isoform 1
• NCBI Official Synonym Full Names: EGF-like domain 7
• NCBI Official Symbol: Egfl7
• NCBI Official Synonym Symbols: Zneu1; VE-statin
• NCBI Protein Information: epidermal growth factor-like protein 7; NEU1 protein; EGF-like protein 7; NOTCH4-like protein; vascular endothelial statin; vascular endothelial-statin; multiple EGF-like domains protein 7; multiple epidermal growth factor-like domains protein 7
• UniProt Protein Name: Epidermal growth factor-like protein 7
• UniProt Gene Name: Egfl7
• UniProt Synonym Gene Names: Megf7; EGF-like protein 7; Multiple EGF-like domains protein 7; VE-statin
• UniProt Entry Name: EGFL7_MOUSE

Uniprot Description

Function: Regulates vascular tubulogenesis in vivo. Inhibits platelet-derived growth factor (PDGF)-BB-induced smooth muscle cell migration and promotes endothelial cells adhesion to the substrate in vitro. Ref.1 Ref.6

Subcellular location: Secreted Ref.1 Ref.2.

Tissue specificity: Expressed specifically by endothelial cells of the highly vascularized organs heart, lung and kidney. Ref.1 Ref.2

Developmental stage: Expressed early during mouse embryogenesis in the yolk sac mesoderm and in the developing vascular system. At E7.5, it is expressed in the primitive blood islands where the first endothelial cells differentiate. At E10.5 and E13.5 expression is restricted to endothelial cells. Ref.1 Ref.2

Sequence similarities: Contains 2 EGF-like domains.Contains 1 EMI domain.

Sequence caution: The sequence AAF01322.1 differs from that shown. Reason: Erroneous initiation. The sequence AAP74732.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB22222.1 differs from that shown. Reason: Erroneous initiation.