Mouse EGF Like Domain Protein, Multiple 7 (EGFL7) RTU ELISA Kit | EGFL7 rtu elisa kit

Mouse EGF Like Domain Protein, Multiple 7 (EGFL7) ELISA Kit

Cat. Num. AAA459005

• Gene Names: Egfl7; Zneu1; VE-statin
• Reactivity: Mouse
• Synonyms: EGF Like Domain Protein; Multiple 7 (EGFL7); Mouse EGF Like Domain Protein; Multiple 7 (EGFL7) ELISA Kit; ZNEU1; MEGF7; Zneu1; Vascular endothelial statin; EGF Like Domain Containing 7; Epidermal growth factor-like protein 7; Multiple epidermal growth factor-like domains protein 7; EGFL7 rtu elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of EGFL7. No significant cross-reactivity or interference between EGFL7 and analogues was observed.
• Sequence Length: 278

• Samples: Serum, plasma, tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.2-2000pg/mL
• Sensitivity: < 15.5pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level EGFL7 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level EGFL7 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for EGFL7 rtu elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of EGFL7 in mouse serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to EGFL7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to EGFL7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EGFL7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of EGFL7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 257096020
• NCBI GeneID: 353156
• NCBI Accession #: NP_001158036.1
• NCBI GenBank Nucleotide #: NM_001164564.1
• UniProt Accession #: Q9QXT5; Q6XD35; Q9DCP5
• Molecular Weight: 28,305 Da
• NCBI Official Full Name: epidermal growth factor-like protein 7 isoform 1
• NCBI Official Synonym Full Names: EGF-like domain 7
• NCBI Official Symbol: Egfl7
• NCBI Official Synonym Symbols: Zneu1; VE-statin
• NCBI Protein Information: epidermal growth factor-like protein 7
• UniProt Protein Name: Epidermal growth factor-like protein 7
• Protein Family: Epidermal growth factor-like protein
• UniProt Gene Name: Egfl7
• UniProt Synonym Gene Names: Megf7; EGF-like protein 7; Multiple EGF-like domains protein 7; VE-statin

Uniprot Description

Regulates vascular tubulogenesis in vivo. Inhibits platelet-derived growth factor (PDGF)-BB-induced smooth muscle cell migration and promotes endothelial cell adhesion to the extracellular matrix and angiogenesis.