Erythropoietin Active Protein | EPO active protein

Erythropoietin, 28-193aa, Human, His tag, Baculovirus (Bioactivity Validated)

Cat. Num. AAA206227

• Gene Names: EPO; EP; MVCD2
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: Erythropoietin; 28-193aa; Human; His tag; Baculovirus (Bioactivity Validated); EPO; EP; MVCD2; EPO active protein

• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALRAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDRLEHH HHHH
• Sequence Length: 193

• Applicable Applications for EPO active protein: SDS-PAGE
• Tag Information: His-tag
• Predicted N terminal: Ala28
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Biological Activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is equal or less than 0.5ng/ml.
• Preparation and Storage: Can be stored at 4°C short term (1-2 weeks).; For long term storage, aliquot and store at -20°C or -70°C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for EPO active protein

EPO, also known as erythropoietin, is a glycoprotein hormone in the type I cytokine family and is related to thrombopoietin. Its three N-glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species. It is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules. This protein can be found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. It also has neuroprotective activity against a variety of potential brain injuries and anti-apoptotic functions in several tissue types. Recombinant human EPO, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for EPO active protein

Cardiovascular; Angiogenesis

References

Koury, M.J., et al. (2005) Exp. Hematol. 33:1263.; Jelkmann W., et al. (2007) Eur J Haematol. 78:183-205

NCBI and Uniprot Product Information

• NCBI GI #: 62240997
• NCBI GeneID: 2056
• NCBI Accession #: NP_000790
• NCBI GenBank Nucleotide #: NM_000799.3
• UniProt Accession #: P01588; Q2M2L6; Q549U2; Q9UDZ0; Q9UEZ5; Q9UHA0
• Molecular Weight: 19.5kDa (174aa) 28-40KDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: erythropoietin
• NCBI Official Synonym Full Names: erythropoietin
• NCBI Official Symbol: EPO
• NCBI Official Synonym Symbols: EP; MVCD2
• NCBI Protein Information: erythropoietin
• UniProt Protein Name: Erythropoietin
• Protein Family: Erythropoietin
• UniProt Gene Name: EPO

NCBI Description

This gene encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The encoded protein is mainly synthesized in the kidney, secreted into the blood plasma, and binds to the erythropoietin receptor to promote red blood cell production, or erythropoiesis, in the bone marrow. Expression of this gene is upregulated under hypoxic conditions, in turn leading to increased erythropoiesis and enhanced oxygen-carrying capacity of the blood. Expression of this gene has also been observed in brain and in the eye, and elevated expression levels have been observed in diabetic retinopathy and ocular hypertension. Recombinant forms of the encoded protein exhibit neuroprotective activity against a variety of potential brain injuries, as well as antiapoptotic functions in several tissue types, and have been used in the treatment of anemia and to enhance the efficacy of cancer therapies. [provided by RefSeq, Aug 2017]

Uniprot Description

Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.